dc.creatorRojas, Bruno Ezequiel
dc.creatorHartman, Matias Daniel
dc.creatorFigueroa, Carlos Maria
dc.creatorIglesias, Alberto Alvaro
dc.date.accessioned2021-10-28T13:37:10Z
dc.date.accessioned2022-10-15T07:35:42Z
dc.date.available2021-10-28T13:37:10Z
dc.date.available2022-10-15T07:35:42Z
dc.date.created2021-10-28T13:37:10Z
dc.date.issued2020-12
dc.identifierRojas, Bruno Ezequiel; Hartman, Matias Daniel; Figueroa, Carlos Maria; Iglesias, Alberto Alvaro; Proteolytic cleavage of Arabidopsis thaliana phosphoenolpyruvate carboxykinase-1 modifies its allosteric regulation; Oxford University Press; Journal of Experimental Botany; 72; 7; 12-2020; 2514-2524
dc.identifier0022-0957
dc.identifierhttp://hdl.handle.net/11336/145313
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4361057
dc.description.abstractPhosphoenolpyruvate carboxykinase (PEPCK) plays a crucial role in gluconeogenesis. In this work, we analyze the proteolysis of Arabidopsis thaliana PEPCK1 (AthPEPCK1) in germinating seedlings. We found that the amount of AthPEPCK1 protein peaks at 24-48 h post-imbibition. Concomitantly, we observed shorter versions of AthPEPCK1, putatively generated by metacaspase-9 (AthMC9). To study the impact of AthMC9 cleavage on the kinetic and regulatory properties of AthPEPCK1, we produced truncated mutants based on the reported AthMC9 cleavage sites. The Δ19 and Δ101 truncated mutants of AthPEPCK1 showed similar kinetic parameters and the same quaternary structure as the wild type. However, activation by malate and inhibition by glucose 6-phosphate were abolished in the Δ101 mutant. We propose that proteolysis of AthPEPCK1 in germinating seedlings operates as a mechanism to adapt the sensitivity to allosteric regulation during the sink-to-source transition.
dc.languageeng
dc.publisherOxford University Press
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/eraa583/6033732
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jxb/eraa583
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectARABIDOPSIS THALIANA
dc.subjectGLUCONEOGENESIS
dc.subjectMETACASPASE
dc.subjectPHOSPHOENOLPYRUVATE CARBOXYKINASE
dc.subjectPROTEOLYSIS
dc.subjectSEEDLINGS
dc.titleProteolytic cleavage of Arabidopsis thaliana phosphoenolpyruvate carboxykinase-1 modifies its allosteric regulation
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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