dc.creator | Centorbi, Hugo José | |
dc.creator | Silva, Humberto de Jesus | |
dc.date.accessioned | 2022-06-10T12:57:08Z | |
dc.date.accessioned | 2022-10-15T07:26:29Z | |
dc.date.available | 2022-06-10T12:57:08Z | |
dc.date.available | 2022-10-15T07:26:29Z | |
dc.date.created | 2022-06-10T12:57:08Z | |
dc.date.issued | 2008-02 | |
dc.identifier | Centorbi, Hugo José; Silva, Humberto de Jesus; Slowly utilized carbon sources enhance botulinic specific toxicity by co-culture of Clostridium argentinense with the non-pathogen Pseudomonas mendocina; Springer; World Journal of Microbiology; 24; 2-2008; 1823-1828 | |
dc.identifier | 0959-3993 | |
dc.identifier | http://hdl.handle.net/11336/159461 | |
dc.identifier | 1573-0972 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4360258 | |
dc.description.abstract | The growth and botulinic toxin production of Clostridium argentinense G 89 HT in co-culture with Pseudomonas mendocina were studied using two culture systems with carbon sources of rapid and slow utilization. Growth with glucose in homogeneous co-culture presented a dual-phase progression, with the toxin produced in the slow-growing phase. The extended 50 h growth period of the second phase at low specific growth rate was attributed to the combined metabolization of glucose and a complex carbon source of the alginate type produced by P. mendocina under strongly reducing conditions. With dextrin, the co-culture grew at the lower specific growth rate (μ = 0.03 h−1) for a period lasting 80 h. This fully enhanced the production of toxin with a specific toxicity 2.5 times higher than with glucose in a homogeneous system and 10.7 higher than that of C. argentinense G 89 HT single culture. The heterogeneous co-culture obtained with a dialysis membrane physically separating both bacteria (thereby eliminating the metabolic interaction) produced the lowest levels of growth and toxin of all the cases analyzed. | |
dc.language | eng | |
dc.publisher | Springer | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11274-008-9676-8 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s11274-008-9676-8 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | CARBON SOURCES | |
dc.subject | CLOSTRIDIUM ARGENTINENSE | |
dc.subject | CO-CULTURE-BOTULINIC TOXIN | |
dc.subject | PSEUDOMONAS MENDOCINA | |
dc.title | Slowly utilized carbon sources enhance botulinic specific toxicity by co-culture of Clostridium argentinense with the non-pathogen Pseudomonas mendocina | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |