dc.creator | Ben Achour, Nahed | |
dc.creator | Power, Pablo | |
dc.creator | Mercuri, Pablo Alberto | |
dc.creator | Moussa, Mohamed Ben | |
dc.creator | Moreno, Galleni | |
dc.creator | Omrane. Belhadj | |
dc.date.accessioned | 2020-06-23T15:39:33Z | |
dc.date.accessioned | 2022-10-15T07:10:23Z | |
dc.date.available | 2020-06-23T15:39:33Z | |
dc.date.available | 2022-10-15T07:10:23Z | |
dc.date.created | 2020-06-23T15:39:33Z | |
dc.date.issued | 2012-03 | |
dc.identifier | Ben Achour, Nahed; Power, Pablo; Mercuri, Pablo Alberto; Moussa, Mohamed Ben; Moreno, Galleni; et al.; First detection of a transferable bla CTX-M-14b gene in a Klebsiella pneumoniae clinical isolate from Tunisia and analysis of its genetic context; Inst Microbiologia; Annals Of Microbiology; 62; 4; 3-2012; 1737-1742 | |
dc.identifier | 1590-4261 | |
dc.identifier | http://hdl.handle.net/11336/107928 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4358937 | |
dc.description.abstract | Klebsiella pneumoniae ML2508 was isolated from a patient at the surgery unit of the Military Hospital (Hôpital Militaire de Tunis), Tunisia. It was identified as a producer of extended-spectrum β-lactamases (ESBLs) by the double-disk synergy test. The β-lactamases produced by the strain were characterized by isoelectric focusing, determination of the specific activities against penicillins and cephalosporins, determination of the inhibitory concentration required to inhibit 50% of enzyme activity (IC50), and the inhibition effect of EDTA on putative metallo-β- lactamases. The crude extract of K. pneumoniae ML2508 contains five different β-lactamases with pI 5.5, 7.3, 7.6, 8.1, and 8.6. Only the β-lactamase with pI 8.1 was transferred by transformation and conjugation experiments. Molecular characterization of these genes was performed by PCR and sequencing. The four chromosomal β-lactamases are TEM (pI 5.5), 2 SHV (pI 7.3 and 7.6), and CTX-M-28 (pI 8.6). The β-lactamase with pI 8.1 was encoded by blaCTX-M-14b gene located on a 50-kb highly conjugative plasmid. The study of the genetic context of blaCTX-M-14b was realized by PCR-mapping and DNA sequencing. A novel variant of tnpISEcp1 designated ISEcp1C was detected upstream of the gene. | |
dc.language | eng | |
dc.publisher | Inst Microbiologia | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13213-012-0430-y | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s13213-012-0430-y | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | b-lactamase | |
dc.subject | PCR-mapping | |
dc.subject | DNA sequencing | |
dc.title | First detection of a transferable bla CTX-M-14b gene in a Klebsiella pneumoniae clinical isolate from Tunisia and analysis of its genetic context | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |