dc.contributor | Bramajo, Pablo | |
dc.contributor | Cabllero, Paul | |
dc.contributor | Ceriani, Silvina | |
dc.creator | Stupniki, Sofia | |
dc.creator | Bayasgalan, Tsogbadrakh | |
dc.creator | Kovács, Adrienn | |
dc.creator | Csemer, Andrea | |
dc.creator | Szentesi, Peter | |
dc.creator | Pocsai, Krisztina | |
dc.creator | Dionisio, Leonardo Raul | |
dc.creator | Spitzmaul, Guillermo Federico | |
dc.creator | Pal, Bálazs | |
dc.date.accessioned | 2022-04-27T17:48:21Z | |
dc.date.accessioned | 2022-10-15T06:44:13Z | |
dc.date.available | 2022-04-27T17:48:21Z | |
dc.date.available | 2022-10-15T06:44:13Z | |
dc.date.created | 2022-04-27T17:48:21Z | |
dc.date.issued | 2020 | |
dc.identifier | KCNQ4 in the reticular activating system (RAS): contribution to the circadian rhythm modulation; XXXV Reunión Anual Sociedad Argentina de Investigación en Neurociencia 2020; Argentina; 2020; 282-282 | |
dc.identifier | http://hdl.handle.net/11336/155895 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4356651 | |
dc.description.abstract | The M-current is a voltage-gated potassium current generated by channels composed by KCNQ2-5 subunits. The pedunculopontine nucleus (PPN) is part of the Reticular Activating System (RAS), associated with sleep regulation. As little is known about the composition, subcellular location and physiological implication of the M-current in PPN, our aim was to demonstrate the presence of KCNQ4 in the PPN, and its contribution to the neuronal function of this nucleus. We used a transgenic mouse lacking KCNQ4 expression (KO) and one with fluorescent-labeled cholinergic neurons (tdTomatoStop+ChAT::Cre). Using qPCR, immunofluorescence and electrophysiology on brain slices, we demonstrated that only a subpopulation of cholinergic neurons (around 27%), located on the external limits of the PPN has KCNQ4-mediated M-current. We also found that KCNQ4 regulates the expression of other KCNQ subunits. In KO mice, the expression profile changed drastically respect with the WT: Kcnq2 expression decreased, Kcnq3 increase and Kcnq5 disappeared. To study the influence of KCNQ4 on circadian rhythm we used behavioral testing. KO mice exhibited alterations in the activity cycles showing a higher sensitivity to changes in the light-darkness cycles. In summary, we found that some PPN cholinergic neurons have KCNQ4-dependent M-current and this subunit contributes to modulate the circadian rhythm. Since the PPN is affected in certain neurological diseases, KCNQ4 might be a potential pharmacological target. | |
dc.language | eng | |
dc.publisher | Sociedad Argentina de Investigación en Neurociencia | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.saneurociencias.org.ar/congresos-san/ | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | EBook Sociedad Argentina de Investigación en Neurociencia 2020 | |
dc.subject | KNCQ4 | |
dc.subject | PPN | |
dc.subject | circadian rhythm | |
dc.subject | RAS | |
dc.title | KCNQ4 in the reticular activating system (RAS): contribution to the circadian rhythm modulation | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | info:eu-repo/semantics/conferenceObject | |
dc.type | info:ar-repo/semantics/documento de conferencia | |