dc.creatorMazorra Morales, Luis Miguel
dc.creatorSenn, María Eugenia
dc.creatorGergoff Grozeff, Gustavo Esteban
dc.creatorFanello, Diego Darío
dc.creatorCarrión, Cristian Antonio
dc.creatorNúñez, Miriam
dc.creatorBishop, Gerard James
dc.creatorBartoli, Carlos Guillermo
dc.date.accessioned2020-04-03T17:54:48Z
dc.date.accessioned2022-10-15T06:36:15Z
dc.date.available2020-04-03T17:54:48Z
dc.date.available2022-10-15T06:36:15Z
dc.date.created2020-04-03T17:54:48Z
dc.date.issued2014-01
dc.identifierMazorra Morales, Luis Miguel; Senn, María Eugenia; Gergoff Grozeff, Gustavo Esteban; Fanello, Diego Darío; Carrión, Cristian Antonio; et al.; Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 74; 322; 1-2014; 315-322
dc.identifier0981-9428
dc.identifierhttp://hdl.handle.net/11336/101900
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4355989
dc.description.abstractPlant steroid hormones brassinosteroids (BRs) and the gaseous hormone ethylene (ET) alter the ascorbic acid - glutathione (AA-GSH) levels in tomato (Solanum lycopersicum L.) plants. The interaction of these hormones in regulating antioxidant metabolism is however unknown. The combined use of genetics (BR mutants) and chemical application (BR/ET-related chemicals) shows that BRs and ET signalling pathways interact, to regulate leaf AA content and synthesis. BR deficient (dx) leaves display low total AA but BR-accumulating (35S:D) leaves show normal total AA content. Leaves with either BR levels lower or higher than wild type plants showed a higher oxidised AA redox state. The activity of L galactono-1,4-lactone dehydrogenase (L-GalLDH), the mitochondrial enzyme that catalyses the last step in AA synthesis is lower in dx and higher in 35S:D plants. BR-deficient mutants show higher ET production but it is restored to normal levels when BR content is increased in 35S:D plants. Suppression of ET signalling using 1-methylcyclopropene in dx and 35S:D plants restored leaf AA content and L-GalLDH activity, to the values observed in wild type. The suppression of ET action in dx and 35S:D leaves leads to the respective decreasing and increasing respiration, indicating an opposite response compared to AA synthesis. This inverse relationship is lacking in ET suppressed dx plants in response to external BRs. The modifications in the in vivo activity of L-GalLDH activity do not correlate with changes in the level of the enzyme. Taken together, these data suggest that ET suppresses and BRs promote AA synthesis and accumulation.
dc.languageeng
dc.publisherElsevier France-editions Scientifiques Medicales Elsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813004166
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plaphy.2013.11.021
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntioxidants
dc.subjectAscorbic acid
dc.subjectBrassinosteroids
dc.subjectEthylene
dc.subjectLeaves
dc.subjectRespiration
dc.subjectTomato
dc.titleImpact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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