dc.creator | Bottero, Daniela | |
dc.creator | Zurita, Maria Eugenia | |
dc.creator | Gaillard, María Emilia | |
dc.creator | Carriquiriborde, Francisco Pablo | |
dc.creator | Martin Aispuro, Pablo | |
dc.creator | Elizagaray, Maia Lina | |
dc.creator | Bartel, Erika Belén | |
dc.creator | Castuma, Celina | |
dc.creator | Hozbor, Daniela Flavia | |
dc.date.accessioned | 2020-02-01T02:43:47Z | |
dc.date.accessioned | 2022-10-15T05:22:30Z | |
dc.date.available | 2020-02-01T02:43:47Z | |
dc.date.available | 2022-10-15T05:22:30Z | |
dc.date.created | 2020-02-01T02:43:47Z | |
dc.date.issued | 2018-10 | |
dc.identifier | Bottero, Daniela; Zurita, Maria Eugenia; Gaillard, María Emilia; Carriquiriborde, Francisco Pablo; Martin Aispuro, Pablo; et al.; Outer-Membrane-Vesicle-Associated O Antigen, a Crucial Component for Protecting Against Bordetella parapertussis Infection; Frontiers Media S.A.; Frontiers in Immunology; 9; 10-2018; 1-12 | |
dc.identifier | 1664-3224 | |
dc.identifier | http://hdl.handle.net/11336/96469 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4349296 | |
dc.description.abstract | Bordetella parapertussis is a respiratory-disease pathogen producing symptomatology similar to that of pertussis but of underestimated incidence and with no specific vaccine existing. We recently designed a vaccine candidate from B. parapertussis outer-membrane vesicles (OMVs) that proved to be safe and protective in a murine-infection model. Based on protection recently reported for the B. parapertussis O antigen in aqueous solution, we assessed here whether the B. parapertussis O-antigen-containing lipopolysaccharide (BppLPS-O+) embedded in the membranes, as present in B. parapertussis-derived OMVs (OMVs(Bpp-LPS-O+)), was the component responsible for that previously observed protection by OMVs. By performing a comparative study with OMVs from a human strain with undetectable O antigen (OMVs(Bpp-LPS-O-)), we demonstrated that the OMVs(Bpp-LPS-O+), but not the OMVs(Bpp-LPS-O-), protected mice against sublethal B. parapertussis infections. Indeed, the B. parapertussis loads were significantly reduced in the lungs of OMVs(Bpp-LPS-O+) -vaccinated animals, with the CFUs recovered being decreased by 4 log units below those detected in the non-immunized animals or in the animals treated with the OMVs(Bpp-LPS-O-), (p < 0.001). We detected that the OMVs(Bpp-LPS-O+) induced IgG antibodies against B. parapertussis whole-cell lysates, which immunocomponents recognized, among others, the O antigen and accordingly conferred protection against B. parapertussis infection, as observed in in-vivo-passive-transfer experiments. Of interest was that the OMVs(Bpp-LPS-O+) -generated sera had opsonophagocytic and bactericidal capabilities that were not detected with the OMVs(Bpp-LPS-O-)-induced sera, suggesting that those activities were involved in the clearance of B. parapertussis. Though stimulation of cultured spleen cells from immunized mice with formulations containing the O antigen resulted in gamma interferon (IFN-γ) and interleukin-17 production, spleen cells from OMVs(Bpp-LPS-O+) -immunized mice did not significantly contribute to the observed protection against B. parapertussis infection. The protective capability of the B. parapertussis O antigen was also detected in formulations containing both the OMVs derived from B. pertussis and purified BppLPS-O+. This combined formulation protected mice against B. pertussis along with B. parapertussis. | |
dc.language | eng | |
dc.publisher | Frontiers Media S.A. | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fimmu.2018.02501/full | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fimmu.2018.02501 | |
dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | BORDETELLA PARAPERTUSSIS | |
dc.subject | LIPOPOLYSACHARIDES | |
dc.subject | O-ANTIGEN | |
dc.subject | OUTER-MEMBRANE VESICLES | |
dc.subject | PROTECTION | |
dc.title | Outer-Membrane-Vesicle-Associated O Antigen, a Crucial Component for Protecting Against Bordetella parapertussis Infection | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |