dc.creatorLevy, Ivana Karina
dc.creatorNeme Tauil, Ricardo Martin
dc.creatorValacco, Maria Pia
dc.creatorMoreno, Silvia Margarita
dc.creatorSiracusano, Gabriela Silvana
dc.creatorMaier, Marta Silvia
dc.date.accessioned2020-02-21T20:54:42Z
dc.date.accessioned2022-10-15T05:07:09Z
dc.date.available2020-02-21T20:54:42Z
dc.date.available2022-10-15T05:07:09Z
dc.date.created2020-02-21T20:54:42Z
dc.date.issued2018-12
dc.identifierLevy, Ivana Karina; Neme Tauil, Ricardo Martin; Valacco, Maria Pia; Moreno, Silvia Margarita; Siracusano, Gabriela Silvana; et al.; Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap); Elsevier Science; Microchemical Journal; 143; 12-2018; 457-466
dc.identifier0026-265X
dc.identifierhttp://hdl.handle.net/11336/98350
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4348070
dc.description.abstractIn this paper, we report the study of proteinaceous binders in samples from a South American Colonial 18th century painting using two complementary mass spectrometry techniques. Before restoration, seven micro-samples were extracted from representative colors of one of the mural paintings located inside the church of Our Lady of Copacabana de Andamarca built in 1723 in Bolivia. Previous analysis by gas chromatography (GC) of the amino acid derivatives of the extracted protein fraction of three of the samples, suggested the presence of animal glue and egg. In this work, we introduce a methodology that combines a protein extraction procedure with a typical treatment for analysis of proteins as tryptic peptides by mass spectrometry. For this study, we applied in the first stage, MALDI-TOF/MS and then, LC-ESI/MS (Orbitrap), an ultrahigh resolution mass spectrometry, to achieve more reliability in the identification of the protein binders. By LC-ESI/MS (Orbitrap), we detected several peptides from egg white proteins, particularly ovalbumin, ovotransferrin and lysozyme, and egg yolk proteins, vitellogenin-2 and apolipoprotein B, with high confidence peptides each. In all the samples, the presence of collagen from animal provenance was established by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). To our knowledge, this is the first time that protein binders in Andean paintings are identified without ambiguity by MALDI-TOF/MS and LC-ESI/MS (Orbitrap) mass spectrometry using a proteomic approach.
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X17308469
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.microc.2018.07.030
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCOLONIAL MURAL PAINTINGS
dc.subjectEGG TEMPERA TECHNIQUE
dc.subjectLC-ESI/MS (ORBITRAP)
dc.subjectMALDI-TOF/MS
dc.subjectPROTEINACEOUS BINDERS
dc.subjectPROTEOMIC TOOLS
dc.titleInvestigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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