dc.creator | He, Weiguo | |
dc.creator | Elizondo Riojas, Miguel Angel | |
dc.creator | Li, Xin | |
dc.creator | Lokesh, Ganesh Lakshmana Rao | |
dc.creator | Somasunderam, Anoma | |
dc.creator | Thiviyanathan, Varatharasa | |
dc.creator | Volk, David E. | |
dc.creator | Durland, Ross H. | |
dc.creator | Englehardt, Johnnie | |
dc.creator | Cavasotto, Claudio Norberto | |
dc.creator | Gorenstein, David G. | |
dc.date.accessioned | 2019-01-15T18:58:26Z | |
dc.date.accessioned | 2022-10-15T04:48:45Z | |
dc.date.available | 2019-01-15T18:58:26Z | |
dc.date.available | 2022-10-15T04:48:45Z | |
dc.date.created | 2019-01-15T18:58:26Z | |
dc.date.issued | 2012-10 | |
dc.identifier | He, Weiguo; Elizondo Riojas, Miguel Angel; Li, Xin; Lokesh, Ganesh Lakshmana Rao; Somasunderam, Anoma; et al.; X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding; American Chemical Society; Biochemistry; 51; 42; 10-2012; 8321-8323 | |
dc.identifier | 0006-2960 | |
dc.identifier | http://hdl.handle.net/11336/68063 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4346651 | |
dc.description.abstract | By combining pseudorandom bead-based aptamer libraries with conjugation chemistry, we have created next-generation aptamers, X-aptamers (XAs). Several X-ligands can be added in a directed or random fashion to the aptamers to further enhance their binding affinities for the target proteins. Here we describe the addition of a drug (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), demonstrated to bind to CD44-HABD, to a complete monothioate backbone-substituted aptamer to increase its binding affinity for the target protein by up to 23-fold, while increasing the drug's level of binding 1-million fold. | |
dc.language | eng | |
dc.publisher | American Chemical Society | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/bi300471d | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/bi300471d | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | X-aptamers | |
dc.subject | Structure-based Drug Design | |
dc.subject | Molecular Dynamics | |
dc.subject | CD44 | |
dc.title | X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |