dc.creatorZavala, Agustín
dc.creatorKovacec, Verónica Ivana
dc.creatorLevín, Gustavo
dc.creatorMoglioni, Albertina Gladys
dc.creatorMiranda, Maria Victoria
dc.creatorGarcía, Ernesto
dc.creatorBonofiglio, Laura
dc.creatorMollerach, Marta Eugenia
dc.date.accessioned2018-03-28T16:42:03Z
dc.date.accessioned2022-10-15T04:46:19Z
dc.date.available2018-03-28T16:42:03Z
dc.date.available2022-10-15T04:46:19Z
dc.date.created2018-03-28T16:42:03Z
dc.date.issued2017-01
dc.identifierZavala, Agustín; Kovacec, Verónica Ivana; Levín, Gustavo; Moglioni, Albertina Gladys; Miranda, Maria Victoria; et al.; Screening assay for inhibitors of a recombinant Streptococcus pneumoniae UDP-glucose pyrophosphorylase; Taylor & Francis; Journal of Enzyme Inhibition and Medicinal Chemistry; 32; 1; 1-2017; 203-207
dc.identifier1475-6366
dc.identifierhttp://hdl.handle.net/11336/40381
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4346462
dc.description.abstractThe UDP-glucose pyrophosphorylase of Streptococcus pneumoniae (GalUSpn) is absolutely required for the biosynthesis of capsular polysaccharide, the sine qua non virulence factor of pneumococcus. Since the eukaryotic enzymes are completely unrelated to their prokaryotic counterparts, we propose that the GalU enzyme is a critical target to fight the pneumococcal disease. A recombinant GalUSpn was overexpressed and purified. An enzymatic assay that is rapid, sensitive and easy to perform was developed. This assay was appropriate for screening chemical libraries for searching GalU inhibitors. This work represents a fundamental step in the exploration of novel antipneumococcal drugs.
dc.languageeng
dc.publisherTaylor & Francis
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/14756366.2016.1247055
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/14756366.2016.1247055
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntibacterial Target
dc.subjectPneumococcus
dc.subjectUtp:Glucose 1-Phosphate Uridylyltransferase
dc.subjectVirulence Inhibitors
dc.titleScreening assay for inhibitors of a recombinant Streptococcus pneumoniae UDP-glucose pyrophosphorylase
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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