dc.creator | Lopez Bergami, Pablo Roberto | |
dc.creator | Gomez, Karina Andrea | |
dc.creator | Levy, Gabriela Vanesa | |
dc.creator | Grippo, Vanina | |
dc.creator | Baldi, Alberto | |
dc.creator | Levin, Mariano Jorge | |
dc.date.accessioned | 2019-07-18T21:08:43Z | |
dc.date.accessioned | 2022-10-15T04:25:36Z | |
dc.date.available | 2019-07-18T21:08:43Z | |
dc.date.available | 2022-10-15T04:25:36Z | |
dc.date.created | 2019-07-18T21:08:43Z | |
dc.date.issued | 2005-10 | |
dc.identifier | Lopez Bergami, Pablo Roberto; Gomez, Karina Andrea; Levy, Gabriela Vanesa; Grippo, Vanina; Baldi, Alberto; et al.; The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 142; 1; 10-2005; 140-147 | |
dc.identifier | 0009-9104 | |
dc.identifier | http://hdl.handle.net/11336/79866 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4344950 | |
dc.description.abstract | BALB/c mice immunized with recombinant Trypanosoma cruzi ribosomal P2β protein (TcP2β) develop a strong and specific antibody response against its 13 residue-long C-terminal epitope (peptide R13: EEEDDDMGFGLFD) that has a concomitant β1-adrenergic stimulating activity. However, other animals that undergo similar immunizations seem tolerant to this epitope. To evaluate further the antibody response against the ribosomal P proteins, 25 BALB/c and 25 Swiss mice were immunized with TcP2β. From the 50 animals, 31 developed a positive anti-R13 response, whereas 19 were non-responsive. From the 31 anti-R13 positive mice, 25 had anti-R13 antibodies that recognized the discontinuous motif ExDDxGF, and their presence correlated with the recording of supraventricular tachycardia. The other six had anti-R13 antibodies but with a normal electrocardiographic recording. These anti-R13 antibodies recognized the motif DDxGF shared by mammals and T. cruzi and proved to be a true anti-P autoantibody because they were similar to those elicited in Swiss, but not in BALB/c mice, by immunization with the C-terminal portion of the mouse ribosomal P protein. Our results show that the recognition of the glutamic acid in position 3 of peptide R13 defines the ability of anti-R13 antibodies to react with the motif AESDE of the second extracellular loop of the β1-adrenergic receptor, setting the molecular basis for their pathogenic β1 adrenoceptor stimulating activity. | |
dc.language | eng | |
dc.publisher | Wiley Blackwell Publishing, Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1809475/ | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-2249.2005.02885.x | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2249.2005.02885.x | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | Β1-ADRENERGIC RECEPTOR | |
dc.subject | PATHOGENIC ANTIBODIES | |
dc.subject | RIBOSOMAL P PROTEINS | |
dc.subject | TRYPANOSOMA CRUZI | |
dc.title | The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |