dc.creatorCarpaneto Freixas, Agustín Eduardo
dc.creatorMoglie, Marcelo Javier
dc.creatorCastagnola, Tais
dc.creatorSalatino, Lucía
dc.creatorDomene, Sabina
dc.creatorMarcovich, Irina
dc.creatorGallino, Sofia Ludmila
dc.creatorWedemeyer, Carolina
dc.creatorGoutman, Juan Diego
dc.creatorPlazas, Paola Viviana
dc.creatorElgoyhen, Ana Belen
dc.date.accessioned2021-08-04T14:21:25Z
dc.date.accessioned2022-10-15T04:16:45Z
dc.date.available2021-08-04T14:21:25Z
dc.date.available2022-10-15T04:16:45Z
dc.date.created2021-08-04T14:21:25Z
dc.date.issued2020-11
dc.identifierCarpaneto Freixas, Agustín Eduardo; Moglie, Marcelo Javier; Castagnola, Tais; Salatino, Lucía; Domene, Sabina; et al.; Unravelling the molecular players at the cholinergic efferent synapse of the zebrafish lateral line; Society for Neuroscience; Journal of Neuroscience; 11-2020; 1-14
dc.identifier0270-6474
dc.identifierhttp://hdl.handle.net/11336/137761
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4344226
dc.description.abstractThe lateral line (LL) is a sensory system that allows fish and amphibians to detect water currents. LL responsiveness is modulated by efferent neurons which aid to distinguish between external and self-generated stimuli, maintaining sensitivity to relevant cues. One component of the efferent system is cholinergic, the activation of which inhibits afferent activity. LL hair cells (HC) share structural, functional and molecular similarities with those of the cochlea, making them a popular model for studying human hearing and balance disorders. Due to these commonalities, one could propose that the receptor at the LL efferent synapse is a α9α10 nicotinic cholinergic one (nAChR). However, the identities of the molecular players underlying acetylcholine (ACh)-mediated inhibition in the LL remain unknown. Surprisingly, through the analysis of single-cell expression studies and in situ hybridization, we describe that α9, but not α10 subunits, are enriched in zebrafish HC. Moreover, the heterologous expression of zebrafish α9 subunits indicates that homomeric receptors are functional and exhibit robust ACh-gated currents blocked by α-Bungarotoxin and strychnine. In addition, in vivo Ca2+ imaging on mechanically-stimulated zebrafish LL HC show that ACh elicits a decrease in evoked Ca2+ signals, irrespective of HC polarity. This effect is blocked by both α-Bungarotoxin and apamin, indicating coupling of ACh-mediated effects to SK potassium channels. Our results indicate that an α9-containing (α9*) nAChR operates at the zebrafish LL efferent synapse. Moreover, the activation of α9* nAChRs most likely leads to LL HC hyperpolarization served by the activation of Ca2+-dependent SK potassium channels.
dc.languageeng
dc.publisherSociety for Neuroscience
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.1772-20.2020
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectefferent
dc.subjectnicotinic receptor
dc.subjectzebrafish
dc.subjectlateral line
dc.titleUnravelling the molecular players at the cholinergic efferent synapse of the zebrafish lateral line
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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