An ACTH-activated protein tyrosine phosphatase (PTP) is modulated by PKA-mediated phosphorylation

dc.creatorPaz, Cristina del Valle
dc.creatorCornejo Maciel, Maria Fabiana
dc.creatorPoderoso, Cecilia
dc.creatorGorostizaga, Alejandra Beatriz
dc.creatorPodesta, Ernesto Jorge
dc.date.accessioned2018-03-19T17:27:14Z
dc.date.accessioned2022-10-15T03:54:32Z
dc.date.available2018-03-19T17:27:14Z
dc.date.available2022-10-15T03:54:32Z
dc.date.created2018-03-19T17:27:14Z
dc.date.issued2000-11
dc.identifierPaz, Cristina del Valle; Cornejo Maciel, Maria Fabiana; Poderoso, Cecilia; Gorostizaga, Alejandra Beatriz; Podesta, Ernesto Jorge; An ACTH-activated protein tyrosine phosphatase (PTP) is modulated by PKA-mediated phosphorylation; Taylor; Endocrine Research; 26; 4; 11-2000; 609-614
dc.identifier0743-5800
dc.identifierhttp://hdl.handle.net/11336/39236
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4342467
dc.description.abstractIn adrenal cortex, ACTH regulation of steroidogenesis depends on PKA-dependent serine/threonine phosphorylation and also on the activity of protein tyrosine phosphatases (PTPs). In addition, ACTH increases total PTPs involving at least three soluble PTPs (50, 82 and 115 kDa). Serine/threonine phosphorylation of these enzymes themselves could be a regulatory mechanism of their activity since the increase of total PTP activity is dependent on PKA-activation. In this report we analyzed the effect of in vitro phospho-dephosphorylation processes on the activity displayed by the ACTH-activated PTP of 115 kDa. Using an in-gel PTP assay we demonstrate that dephosphorylation catalyzed by potato acid phosphatase (PAP) reduces the activity of the 115 kDa PTP present in ZF from ACTH-treated animals and PKA-mediated phosphorylation reverses this effect.
dc.languageeng
dc.publisherTaylor
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3109/07435800009048579
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.3109/07435800009048579
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectActh
dc.subjectTyrosine Phosphatase
dc.subjectPka
dc.subjectPhosphorylation
dc.titleAn ACTH-activated protein tyrosine phosphatase (PTP) is modulated by PKA-mediated phosphorylation
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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