dc.creatorBesada, Lucas Nahuel
dc.creatorPeruzzo, Pablo Jose
dc.creatorCortizo, Ana María
dc.creatorCortizo, Maria Susana
dc.date.accessioned2019-09-19T21:13:00Z
dc.date.accessioned2022-10-15T03:28:12Z
dc.date.available2019-09-19T21:13:00Z
dc.date.available2022-10-15T03:28:12Z
dc.date.created2019-09-19T21:13:00Z
dc.date.issued2018-08
dc.identifierBesada, Lucas Nahuel; Peruzzo, Pablo Jose; Cortizo, Ana María; Cortizo, Maria Susana; Preparation, characterization, and in vitro activity evaluation of triblock copolymer-based polymersomes for drugs delivery; Springer; Journal of Nanoparticle Research; 20; 3; 8-2018; 1-12
dc.identifier1388-0764
dc.identifierhttp://hdl.handle.net/11336/83939
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4340092
dc.description.abstractPolymersomes are polymer-based vesicles that form upon hydration of amphiphilic block copolymers and display high stability and durability, due to their mechanical and physical properties. They have hydrophilic reservoirs as well as thick hydrophobic membranes; allowing to encapsulate both watersoluble bioactive agent and hydrophobic drugs. In this study, poly ethylene glycol (PEG3350 and PEG6000) were used as hydrophilic part and poly(vinyl benzoate) (PVBz) as hydrophobic block to synthesize amphiphilic triblock copolymers (PVBz-b-PEG-b-PVBz). Different proportions of hydrophilic/hydrophobic part were assayed in order to obtain polymersomes by solvent injection method. For the synthesis of the copolymers, the initial block of PEG was derived to obtain a macroinitiator through a xanthate functional group (PEGX3 or PEGX6) and the polymerization of vinyl benzoate was carried out through reversible additionfragmentation chain transfer polymerization (RAFT). The structure of PEGX and copolymers was confirmed by Infrared, 1H-NMR and UV-Vis spectrometry, while the average molecular weight (Mw) and polydispersity index (PI) were determined by size exclusion chromatography (SEC). The structures adopted by the copolymers in aqueous solution by self-assembly were investigated using transmission electron microscopy (TEM), dynamic light scattering (DLS) and small-angle X-ray scattering (SAXS). Both techniques confirm that polymersomes were obtained for a fraction of hydrophilic block (f) ≈ 35 ± 10%, with a diameter of 38.3 ± 0.3 nm or 22.5 ± 0.7 nm, as determined by TEM and according to the Mw of the precursor block copolymer. In addition, we analyzed the possible cytotoxicity in view of its potential application as biomedical nanocarrier. The results suggest that polymersomes seem not induce cytotoxicity during the periods of time tested.
dc.languageeng
dc.publisherSpringer
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11051-018-4169-7
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11051-018-4169-7
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCytotoxicity
dc.subjectNanomedicine
dc.subjectPolymersomes
dc.subjectSelfassembly
dc.subjectTriblock Copolymer
dc.titlePreparation, characterization, and in vitro activity evaluation of triblock copolymer-based polymersomes for drugs delivery
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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