dc.creator | Trucco, Verónica Milagros | |
dc.creator | Reinheimer, Jorge Alberto | |
dc.creator | Quiberoni, Andrea del Lujan | |
dc.creator | Suárez, Viviana Beatriz | |
dc.date.accessioned | 2021-08-24T15:47:37Z | |
dc.date.accessioned | 2022-10-15T03:04:09Z | |
dc.date.available | 2021-08-24T15:47:37Z | |
dc.date.available | 2022-10-15T03:04:09Z | |
dc.date.created | 2021-08-24T15:47:37Z | |
dc.date.issued | 2011-04 | |
dc.identifier | Trucco, Verónica Milagros; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Suárez, Viviana Beatriz; Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 110; 4; 4-2011; 935-942 | |
dc.identifier | 1364-5072 | |
dc.identifier | http://hdl.handle.net/11336/138791 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4338038 | |
dc.description.abstract | Aims: The aim of this work was to study the adsorption step of two new temperate bacteriophages (Cb1/204 and Cb1/342) of Lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. Methods and Results: The effect of divalent cations, pH, temperature and cell viability on adsorption step was analysed. The Ca2+ presence was necessary for the phage Cb1/342 but not for the phage Cb1/204. Both phages showed to be stable at pH values between 3 and 8. Their adsorption rates decreased considerably at pH 8 but remained high at acid pH values. The optimum temperatures for the adsorption step were between 30 and 40°C. For the phage Cb1/342, nonviable cells adsorbed a lower quantity of phage particles in comparison with the viable ones, a fact that could be linked to disorganization of phage receptor sites and/or to the physiological cellular state. The isolation of phage-resistant derivatives with good technological properties from the sensitive strains and their relationship with the cell heterogeneity of the strains were also made. Conclusions: Characterization of the adsorption step for the first temperate Lact. delbrueckii phages isolated in Argentina was made, and phage-resistant derivatives of their host strains were obtained. Significance and Impact of the Study: Some phage-resistant derivatives isolated exhibited good technological properties with the prospective to be used at industrial level. | |
dc.language | eng | |
dc.publisher | Wiley Blackwell Publishing, Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1111/j.1365-2672.2011.04945.x | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2011.04945.x | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | DAIRY PROCESSES | |
dc.subject | LACTIC ACID BACTERIA | |
dc.subject | LACTOBACILLUS DELBRUECKII | |
dc.subject | PHAGE RESISTANCE | |
dc.subject | TEMPERATE BACTERIOPHAGES | |
dc.title | Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |