dc.creatorArcon, Nadia
dc.creatorPicchio, Mariano Sergio
dc.creatorFenoy, Ignacio Martín
dc.creatorMoretta, Rosalia Ester
dc.creatorSoto, Ariadna Soledad
dc.creatorPerrone Sibilia, Matias
dc.creatorSánchez, Vanesa Roxana
dc.creatorPrato, Cecilia Arahi
dc.creatorTribulatti, María Virginia
dc.creatorGoldman, Alejandra
dc.creatorMartin, Valentina
dc.date.accessioned2021-12-03T18:45:05Z
dc.date.accessioned2022-10-15T02:47:59Z
dc.date.available2021-12-03T18:45:05Z
dc.date.available2022-10-15T02:47:59Z
dc.date.created2021-12-03T18:45:05Z
dc.date.issued2021-02
dc.identifierArcon, Nadia; Picchio, Mariano Sergio; Fenoy, Ignacio Martín; Moretta, Rosalia Ester; Soto, Ariadna Soledad; et al.; Synergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection; Elsevier; Vaccine; 39; 6; 2-2021; 933-942
dc.identifier0264-410X
dc.identifierhttp://hdl.handle.net/11336/148166
dc.identifier1873-2518
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4336840
dc.description.abstractToxoplasmosis is a zoonotic disease with worldwide prevalence in humans and warm-blooded animal populations. In livestock Toxoplasma gondii is the causal agent of significant economic losses since it can cause abortions in goats and sheep. It is estimated that one third of the world population is infected. Although there are effective therapies for acute infection, these are sometimes poorly tolerated, teratogenic, and have a long administration time. Considering the deficiencies that exist related to the prevention and treatment of toxoplasmosis, the development of a safe and effective vaccine would be extremely valuable in fighting against this infection. In the present work, we characterize for the first time the adjuvant and immunogenic potential of a recombinant profilin protein (rTgPF), in a vaccine formulation alone or in combination with the well-known GRA7 antigen candidate in a murine toxoplasmosis model. Since TgPF acts as a ligand for TLR11 and 12 inducing innate immune responses that promote type 1 adaptive responses, we first study the capacity of the mix rGRA7 + rTgPF to initiate an immune response by evaluating dendritic cell activation. Both rTgPF and rGRA7 induces activation of mouse BMDCs more efficiently than the single proteins, evidenced by increased expression of CD80 and CD86 co-stimulatory proteins and secretion of IL-6, IL-10 and IL-12 cytokines after in vitro stimulation. The sum of the effects of rGRA7 and rTgPF on BMDCs maturation led us to assay them in a vaccination protocol. BALB/c mice vaccinated with this mix elicited a Th1-biased immunity via the induction of lymphocyte proliferation, activation of CD4+T cells and increased IFN-γ production that resulted in enhanced protection against chronic Toxoplama gondii infection. Profilin per se induce only cellular immunity but augments the effect of rGRA7 immune responses when used together, thus allowing us to postulate rTgPF as a potential adjuvant in a protein vaccine.
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://www.sciencedirect.com/science/article/pii/S0264410X20316698?via%3Dihub
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vaccine.2020.12.072
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBMDCS ACTIVATION
dc.subjectGRA7
dc.subjectPROFILIN
dc.subjectTOXOPLASMA GONDII
dc.subjectVACCINE
dc.titleSynergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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