dc.creator | Brunetti, Jesús Emanuel | |
dc.creator | Foscaldi, Sabrina Andrea | |
dc.creator | Quintana, Verónica Mara | |
dc.creator | Scolaro, Luis Alberto | |
dc.creator | Lopez, Nora Mabel | |
dc.creator | Castilla, Viviana | |
dc.date.accessioned | 2020-03-12T19:53:33Z | |
dc.date.accessioned | 2022-10-15T02:45:59Z | |
dc.date.available | 2020-03-12T19:53:33Z | |
dc.date.available | 2022-10-15T02:45:59Z | |
dc.date.created | 2020-03-12T19:53:33Z | |
dc.date.issued | 2018-04 | |
dc.identifier | Brunetti, Jesús Emanuel; Foscaldi, Sabrina Andrea; Quintana, Verónica Mara; Scolaro, Luis Alberto; Lopez, Nora Mabel; et al.; Role of the ERK1/2 signaling pathway in the replication of junín and tacaribe viruses; MDPI; Viruses; 10; 4; 4-2018 | |
dc.identifier | 1999-4915 | |
dc.identifier | http://hdl.handle.net/11336/99345 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4336674 | |
dc.description.abstract | We have previously shown that the infection of cell cultures with the arenaviruses Junín (JUNV), Tacaribe (TCRV), and Pichindé promotes the phosphorylation of mitogen-activated protein kinases (MAPKs) extracellular signal-regulated kinases 1 and 2 (ERK1/2) and that this activation is required for the achievement of a productive infection. Here we examined the contribution of ERK1/2 in early steps of JUNV and TCRV multiplication. JUNV adsorption, internalization, and uncoating were not affected by treatment of cultured cells with U0126, an inhibitor of the ERK1/2 signaling pathway. In contrast, U0126 caused a marked reduction in viral protein expression and RNA synthesis, while JUNV RNA synthesis was significantly augmented in the presence of an activator of the ERK1/2 pathway. Moreover, U0126 impaired the expression of a reporter gene in a TCRV-based replicon system, confirming the ability of the compound to hinder arenavirus macromolecular synthesis. By using a cell-based assay, we determined that the inhibitor did not affect the translation of a synthetic TCRV-like mRNA. No changes in the phosphorylation pattern of the translation factor eIF2α were found in U0126-treated cells. Our results indicate that U0126 impairs viral RNA synthesis, thereby leading to a subsequent reduction in viral protein expression. Thus, we conclude that ERK1/2 signaling activation is required for an efficient arenavirus RNA synthesis. | |
dc.language | eng | |
dc.publisher | MDPI | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1999-4915/10/4/199 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/v10040199 | |
dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | ARENAVIRUS | |
dc.subject | CELL SIGNALING | |
dc.subject | ERK | |
dc.subject | JUNÍN VIRUS | |
dc.subject | REPLICATION | |
dc.subject | TACARIBE VIRUS | |
dc.title | Role of the ERK1/2 signaling pathway in the replication of junín and tacaribe viruses | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |