dc.creator | Silva, Jessica Alejandra | |
dc.creator | de Gregorio, Priscilla Romina | |
dc.creator | Rivero, Guadalupe | |
dc.creator | Abraham, Gustavo Abel | |
dc.creator | Nader Macías, María Elena Fátima | |
dc.date.accessioned | 2021-09-28T11:39:28Z | |
dc.date.accessioned | 2022-10-15T02:32:26Z | |
dc.date.available | 2021-09-28T11:39:28Z | |
dc.date.available | 2022-10-15T02:32:26Z | |
dc.date.created | 2021-09-28T11:39:28Z | |
dc.date.issued | 2021-01 | |
dc.identifier | Silva, Jessica Alejandra; de Gregorio, Priscilla Romina; Rivero, Guadalupe; Abraham, Gustavo Abel; Nader Macías, María Elena Fátima; Immobilization of vaginal Lactobacillus in polymeric nanofibers for its incorporation in vaginal probiotic products; Elsevier Science; European Journal of Pharmaceutical Sciences; 156; 1-2021; 1-13 | |
dc.identifier | 0928-0987 | |
dc.identifier | http://hdl.handle.net/11336/141673 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4335498 | |
dc.description.abstract | Probiotic products require high number of viable and active microorganisms during storage. In this work, the survival of human vaginal Lactobacillus gasseri CRL1320 and Lactobacillus rhamnosus CRL1332 after nanofiber-immobilization by electrospinning with polyvinyl-alcohol, and during storage was evaluated. The optimization of bacterial immobilization and storage conditions using bioprotectors (skim milk-lactose and glycerol) and oxygen-excluding packaging was carried out, compared with lyophilization. After electrospinning, a higher survival rate of L. rhamnosus (93%) compared to L. gasseri (84%) was obtained in nanofibers, with high viable cells (>107 colony-forming unit/g) of the two probiotics in nanofibers stored at -20°C up to 14 days. The storage in oxygen-excluding packaging was an excellent strategy to extend the shelf-life of L. rhamnosus (up to 1.7 × 108 CFU/g) in nanofibers stored at 4°C during 360 days, with no addition of bioprotectives, resulting similar to freeze-dried-cells. L. rhamnosus was successfully incorporated into polymeric hydrophilic nanofibers with a mean diameter of 95 nm. The composite materials were characterized in terms of morphology, and their physicochemical and thermal properties assessed. Nanofiber-immobilized L. rhamnosus cells maintained the inhibition to urogenital pathogens. Thus, polymeric nanofiber-immobilized L. rhamnosus CRL1332 can be included in vaginal probiotic products to prevent or treat urogenital infections. | |
dc.language | eng | |
dc.publisher | Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0928098720303511 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ejps.2020.105563 | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | BACTERIAL IMMOBILIZATION | |
dc.subject | ELECTROSPINNING | |
dc.subject | FREEZE-DRIED | |
dc.subject | LACTOBACILLUS | |
dc.subject | NANOFIBERS | |
dc.subject | VAGINAL PROBIOTIC | |
dc.title | Immobilization of vaginal Lactobacillus in polymeric nanofibers for its incorporation in vaginal probiotic products | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |