dc.creatorPerez, Ana Paula
dc.creatorCasasco, Agustina
dc.creatorSchilrreff, Priscila
dc.creatorDefain Tesoriero, María Victoria
dc.creatorDuempelmann, Luc
dc.creatorPappalardo, Juan Sebastian
dc.creatorAltube, María Julia
dc.creatorHiga, Leticia Herminia
dc.creatorMorilla, María José
dc.creatorPetray, Patricia Beatriz
dc.creatorRomero, Eder Lilia
dc.date.accessioned2020-07-02T15:25:03Z
dc.date.accessioned2022-10-15T01:10:31Z
dc.date.available2020-07-02T15:25:03Z
dc.date.available2022-10-15T01:10:31Z
dc.date.created2020-07-02T15:25:03Z
dc.date.issued2014-07
dc.identifierPerez, Ana Paula; Casasco, Agustina; Schilrreff, Priscila; Defain Tesoriero, María Victoria; Duempelmann, Luc ; et al.; Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes; Dove Press; International Journal of Nanomedicine; 9; 7-2014; 3335-3345
dc.identifier1176-9114
dc.identifierhttp://hdl.handle.net/11336/108650
dc.identifier1178-2013
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4328522
dc.description.abstractIn this work, the in vitro anti-Leishmania activity of photodynamic liposomes made of soybean phosphatidylcholine, sodium cholate, total polar archaeolipids (TPAs) extracted from the hyperhalophile archaea Halorubrum tebenquichense and the photosensitizer zinc phthalocyanine (ZnPcAL) was compared to that of ultradeformable photodynamic liposomes lacking TPAs (ZnPcUDLs). We found that while ZnPcUDLs and ZnPcALs (130 nm mean diameter and ?35 mV zeta potential) were innocuous against promastigotes, a low concentration (0.01 µM ZnPc and 7.6 µM phospholipids) of ZnPcALs irradiated at a very low-energy density (0.2 J/cm2) eliminated L. braziliensis amastigotes from J774 macrophages, without reducing the viability of the host cells. In such conditions, ZnPcALs were harmless for J774 macrophages, HaCaT keratinocytes, and bone marrow-derived dendritic cells. Therefore, topical photodynamic treatment would not likely affect skin-associated lymphoid tissue. ZnPcALs were extensively captured by macrophages, but ZnPcUDLs were not, leading to 2.5-fold increased intracellular delivery of ZnPc than with ZnPcUDLs. Despite mediating low levels of reactive oxygen species, the higher delivery of ZnPc and the multiple (caveolin- and clathrin-dependent plus phagocytic) intracellular pathway followed by ZnPc would have been the reason for the higher antiamastigote activity of ZnPcALs. The leishmanicidal activity of photodynamic liposomal ZnPc was improved by TPA-containing liposomes.
dc.languageeng
dc.publisherDove Press
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099200/
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2147/IJN.S60543
dc.rightshttps://creativecommons.org/licenses/by-nc/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMACROPHAGES
dc.subjectLEISHMANIA AMASTIGOTES
dc.subjectZN INTRACELLULAR DELIVERY
dc.subjectIRRADIATION
dc.titleEnhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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