dc.creatorAvanza, María Victoria
dc.creatorÁlvarez Rivera, Gerardo
dc.creatorCifuentes, Alejandro
dc.creatorMendiola, José A.
dc.creatorIbáñez, Elena
dc.date.accessioned2021-07-29T13:45:11Z
dc.date.accessioned2022-10-15T00:56:44Z
dc.date.available2021-07-29T13:45:11Z
dc.date.available2022-10-15T00:56:44Z
dc.date.created2021-07-29T13:45:11Z
dc.date.issued2021-01
dc.identifierAvanza, María Victoria; Álvarez Rivera, Gerardo; Cifuentes, Alejandro; Mendiola, José A.; Ibáñez, Elena; Phytochemical and functional characterization of phenolic compounds from cowpea (Vigna unguiculata (l.) walp.) obtained by green extraction technologies; Molecular Diversity Preservation International; Agronomy; 11; 1; 1-2021; 1-18
dc.identifier2073-4395
dc.identifierhttp://hdl.handle.net/11336/137312
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4327331
dc.description.abstractThis work presents a green, downstream process, from extraction to phytochemical characterization and bioactivity testing, to obtain and evaluate the functional properties of phenolic compounds from cowpea (Vigna unguiculata (L.) Walp.) seeds and pods. Phenolic-rich extracts were obtained by pressurized liquid extraction (PLE). The main factors affecting the extraction conditions (temperature and solvent) were optimized in order to attain extracts with the highest extraction yield, antioxidant capacity, and total phenolic content. The optimal extraction conditions were 1:1 ethanol:water at 170◦C with one extraction cycle for seeds and three extraction cycles for pods. Phenolic compounds of optimal extract were analyzed by UHPLC-q-TOF-MS/MS (quadrupole-time of flight tandem MS). The obtained PLE-extracts exhibited higher phenolic content and antioxidant activity compared to conventional extraction procedures. The in vitro anti-neurodegenerative potential of extracts was measured through Acetylcholinesterase (AChE) inhibition assay. The results revealed the higher bioactivity observed in cowpea pod samples compared to seed extracts, which might be related to higher levels of quercetin and quercetin glycosides, kaempferol diglucoside, and other tetrahydroxylated flavones and flavonols identified in these samples. These results also provide an added-value benefit to the cultivation of this legume, considering the high potential of cowpea phenolic extracts as nutraceutical and functional ingredients in food formulations.
dc.languageeng
dc.publisherMolecular Diversity Preservation International
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/11/1/162
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/agronomy11010162
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectACETYLCHOLINESTERASE
dc.subjectANTIOXIDANT CAPACITY
dc.subjectCOWPEA
dc.subjectGREEN EXTRACTION
dc.subjectNEUROPROTECTIVE PROPERTIES
dc.subjectPHENOLIC COMPOUNDS
dc.subjectPRESSURIZED LIQUID EXTRACTION
dc.titlePhytochemical and functional characterization of phenolic compounds from cowpea (Vigna unguiculata (l.) walp.) obtained by green extraction technologies
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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