Vitellogenin and Lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker

dc.creatorGarcia, Carlos Fernando
dc.creatorHeras, Horacio
dc.date.accessioned2020-01-22T14:39:30Z
dc.date.accessioned2022-10-15T00:47:21Z
dc.date.available2020-01-22T14:39:30Z
dc.date.available2022-10-15T00:47:21Z
dc.date.created2020-01-22T14:39:30Z
dc.date.issued2012-08
dc.identifierGarcia, Carlos Fernando; Heras, Horacio; Vitellogenin and Lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker; Pergamon-Elsevier Science Ltd; Marine Pollution Bulletin; 64; 8; 8-2012; 1631-1636
dc.identifier0025-326X
dc.identifierhttp://hdl.handle.net/11336/95536
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4326454
dc.description.abstractDuring reproduction vitellogenin (VTG) is transported to vitellogenic oocytes as a precursor of egg yolk lipovitellin (LV). As VTG synthesis is affected by environmental stressors, it is widely used as biomarker in endocrine disruption studies. However, it has seldom been employed to evaluate invertebrate hydrocarbon pollution. An ELISA with anti-LV antibody was developed to evaluate the impact of water-soluble fraction of crude oil (WSF) on Macrobrachium borellii vitellogenesis. Prawn VTG concentration was within the range reported for other crustaceans; LV values were positively correlated with gonadosomatic index (GSI). Females at different vitellogenic stages were exposed to a sub-lethal concentration of WSF for 7 days. Exposed animals with GSI > 7 increased their VTG and LV titer as compared to control organisms (190% and 140%, respectively). VTG levels in M. borellii were upregulated and highly sensitive to WSF exposure. This assay could be employed as a biomarker for freshwater hydrocarbon pollution.
dc.languageeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0025326X12002470
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.marpolbul.2012.05.027
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBIOMARKER
dc.subjectCRUSTACEAN
dc.subjectHYDROCARBON POLLUTION
dc.subjectLIPOVITELLIN
dc.subjectVITELLOGENIN
dc.titleVitellogenin and Lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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