dc.creator | Zang, Xiaoling | |
dc.creator | Monge, Maria Eugenia | |
dc.creator | Gaul, David A. | |
dc.creator | McCarty, Nael A. | |
dc.creator | Stecenko, Arlene | |
dc.creator | Fernández, Facundo M. | |
dc.date.accessioned | 2021-08-19T12:09:03Z | |
dc.date.accessioned | 2022-10-15T00:45:58Z | |
dc.date.available | 2021-08-19T12:09:03Z | |
dc.date.available | 2022-10-15T00:45:58Z | |
dc.date.created | 2021-08-19T12:09:03Z | |
dc.date.issued | 2019-10 | |
dc.identifier | Zang, Xiaoling; Monge, Maria Eugenia; Gaul, David A.; McCarty, Nael A.; Stecenko, Arlene; et al.; Early Detection of Cystic Fibrosis Acute Pulmonary Exacerbations by Exhaled Breath Condensate Metabolomics; American Chemical Society; Journal of Proteome Research; 19; 10-2019; 144-152 | |
dc.identifier | 1535-3893 | |
dc.identifier | http://hdl.handle.net/11336/138500 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4326356 | |
dc.description.abstract | The most common cause of death in cystic fibrosis (CF) patients is progressive lung function decline, which is punctuated by acute pulmonary exacerbations (APEs). A major challenge is to discover biomarkers for detecting an oncoming APE and allow for pre-emptive clinical interventions. Metabolic profiling of exhaled breath condensate (EBC) samples collected from CF patients before, during, and after APEs and under stable conditions (n = 210) was performed using ultraperformance liquid chromatography (UPLC) coupled to Orbitrap mass spectrometry (MS). Negative ion mode MS data showed that classification between metabolic profiles from "pre-APE" (pending APE before the CF patient had any signs of illness) and stable CF samples was possible with good sensitivities (85.7 and 89.5%), specificities (88.4 and 84.1%), and accuracies (87.7 and 85.7%) for pediatric and adult patients, respectively. Improved classification performance was achieved by combining positive with negative ion mode data. Discriminant metabolites included two potential biomarkers identified in a previous pilot study: Lactic acid and 4-hydroxycyclohexylcarboxylic acid. Some of the discriminant metabolites had microbial origins, indicating a possible role of bacterial metabolism in APE progression. The results show promise for detecting an oncoming APE using EBC metabolites, thus permitting early intervention to abort such an event. | |
dc.language | eng | |
dc.publisher | American Chemical Society | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00443 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acs.jproteome.9b00443 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | CYSTIC FIBROSIS | |
dc.subject | EXACERBATION | |
dc.subject | MASS SPECTROMETRY | |
dc.subject | METABOLOMICS | |
dc.title | Early Detection of Cystic Fibrosis Acute Pulmonary Exacerbations by Exhaled Breath Condensate Metabolomics | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |