Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

dc.creatorTakeishi, Kazuki
dc.creatorCollin de I'Hortet, Alexandra
dc.creatorWang, Yang
dc.creatorHanda, Kan
dc.creatorGuzman Lepe, Jorge
dc.creatorMatsubara, Kentaro
dc.creatorMorita, Kazutoyo
dc.creatorJang, Sae
dc.creatorHaep, Nils
dc.creatorFlorentino, Rodrigo M.
dc.creatorYuan, Fangchao
dc.creatorFukumitsu, Ken
dc.creatorTobita, Kimimasa
dc.creatorSun, Wendell
dc.creatorFranks, Jonathan
dc.creatorDelgado, Evan R.
dc.creatorShapiro, Erik M.
dc.creatorFraunhoffer Navarro, Nicolas Alejandro
dc.creatorDuncan, Andrew W.
dc.creatorYagi, Hiroshi
dc.creatorMashimo, Tomoji
dc.creatorFox, Ira J.
dc.creatorSoto Gutierrez, Alejandro
dc.date.accessioned2021-09-16T11:49:21Z
dc.date.accessioned2022-10-15T00:21:24Z
dc.date.available2021-09-16T11:49:21Z
dc.date.available2022-10-15T00:21:24Z
dc.date.created2021-09-16T11:49:21Z
dc.date.issued2020-06
dc.identifierTakeishi, Kazuki; Collin de I'Hortet, Alexandra; Wang, Yang; Handa, Kan; Guzman Lepe, Jorge; et al.; Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells; Cell Press; Cell Reports; 31; 9; 6-2020; 1-30
dc.identifier2211-1247
dc.identifierhttp://hdl.handle.net/11336/140478
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4324200
dc.description.abstractThe availability of an autologous transplantable auxiliary liver would dramatically affect the treatment of liver disease. Assembly and function in vivo of a bioengineered human liver derived from induced pluripotent stem cells (iPSCs) has not been previously described. By improving methods for liver decellularization, recellularization, and differentiation of different liver cellular lineages of human iPSCs in an organ-like environment, we generated functional engineered human mini livers and performed transplantation in a rat model. Whereas previous studies recellularized liver scaffolds largely with rodent hepatocytes, we repopulated not only the parenchyma with human iPSC-hepatocytes but also the vascular system with human iPS-endothelial cells, and the bile duct network with human iPSC-biliary epithelial cells. The regenerated human iPSC-derived mini liver containing multiple cell types was tested in vivo and remained functional for 4 days after auxiliary liver transplantation in immunocompromised, engineered (IL2rg−/−) rats.
dc.languageeng
dc.publisherCell Press
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2211124720306884
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.celrep.2020.107711
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBIOENGINEERED HUMAN LIVER
dc.subjectHUMAN IPS CELLS
dc.subjectHUMAN IPS-BILIARY CELLS
dc.subjectHUMAN IPS-ENDOTHELIAL CELLS
dc.subjectHUMAN IPS-HEPATOCYTES
dc.subjectLIVER MATURATION
dc.subjectMINI HUMAN LIVER
dc.subjectORGAN-MICROENVIRONMENT
dc.subjectTRANSPLANTATION
dc.titleAssembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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