Vasoactive Intestinal Peptide induces glucose and neutral amino acid uptake through mTOR signalling in human cytotrophoblast cells

dc.creatorMerech, Fátima Isabel
dc.creatorSoczewski, Elizabeth Victoria
dc.creatorHauk, Vanesa Cintia
dc.creatorPaparini, Daniel Esteban
dc.creatorRamhorst, Rosanna Elizabeth
dc.creatorVota, Daiana Marina
dc.creatorPerez Leiros, Claudia
dc.date.accessioned2021-01-21T12:59:50Z
dc.date.accessioned2022-10-14T23:47:30Z
dc.date.available2021-01-21T12:59:50Z
dc.date.available2022-10-14T23:47:30Z
dc.date.created2021-01-21T12:59:50Z
dc.date.issued2019-11
dc.identifierMerech, Fátima Isabel; Soczewski, Elizabeth Victoria; Hauk, Vanesa Cintia; Paparini, Daniel Esteban; Ramhorst, Rosanna Elizabeth; et al.; Vasoactive Intestinal Peptide induces glucose and neutral amino acid uptake through mTOR signalling in human cytotrophoblast cells; Nature; Scientific Reports; 9; 1; 11-2019; 17152-17152
dc.identifier2045-2322
dc.identifierhttp://hdl.handle.net/11336/123279
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4321146
dc.description.abstractThe transport of nutrients across the placenta involves trophoblast cell specific transporters modulated through the mammalian target of rapamycin (mTOR). The vasoactive intestinal peptide (VIP) has embryotrophic effects in mice and regulates human cytotrophoblast cell migration and invasion. Here we explored the effect of VIP on glucose and System A amino acid uptake by human trophoblast-derived cells (Swan 71 and BeWo cell lines). VIP activated D-glucose specific uptake in single cytotrophoblast cells in a concentration-dependent manner through PKA, MAPK, PI3K and mTOR signalling pathways. Glucose uptake was reduced in VIP-knocked down cytotrophoblast cells. Also, VIP stimulated System A amino acid uptake and the expression of GLUT1 glucose transporter and SNAT1 neutral amino acid transporter. VIP increased mTOR expression and mTOR/S6 phosphorylation whereas VIP silencing reduced mTOR mRNA and protein expression. Inhibition of mTOR signalling with rapamycin reduced the expression of endogenous VIP and of VIP-induced S6 phosphorylation. Our findings support a role of VIP in the transport of glucose and neutral amino acids in cytotrophoblast cells through mTOR-regulated pathways and they are instrumental for understanding the physiological regulation of nutrient sensing by endogenous VIP at the maternal-foetal interface.
dc.languageeng
dc.publisherNature
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-019-53676-3.pdf
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1038/s41598-019-53676-3
dc.rightshttps://creativecommons.org/licenses/by/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGLUCOSE UPTAKE
dc.subjectCYTOTROPHOBLAST CELLS
dc.subjectVIP
dc.subjectmTOR
dc.titleVasoactive Intestinal Peptide induces glucose and neutral amino acid uptake through mTOR signalling in human cytotrophoblast cells
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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