dc.creatorEspejo, María Sofía
dc.creatorOrlowski, Alejandro
dc.creatorIbañez, Alejandro Martin
dc.creatorDi Mattia, Romina Alejandra
dc.creatorCarrizo Velasquez, Fernanda Elisabeth
dc.creatorRossetti, Noelia
dc.creatorCiancio, Maria Carolina
dc.creatorde Giusti, Verónica Celeste
dc.creatorAiello, Ernesto Alejandro
dc.date.accessioned2021-10-07T14:38:51Z
dc.date.accessioned2022-10-14T23:20:29Z
dc.date.available2021-10-07T14:38:51Z
dc.date.available2022-10-14T23:20:29Z
dc.date.created2021-10-07T14:38:51Z
dc.date.issued2019-11-22
dc.identifierEspejo, María Sofía; Orlowski, Alejandro; Ibañez, Alejandro Martin; Di Mattia, Romina Alejandra; Carrizo Velasquez, Fernanda Elisabeth; et al.; The functional association between the sodium/bicarbonate cotransporter (NBC) and the soluble adenylyl cyclase (sAC) modulates cardiac contractility; Springer Verlag Berlín; Pflugers Archiv-European Journal of Physiology; 472; 1; 22-11-2019; 103-115
dc.identifier0031-6768
dc.identifierhttp://hdl.handle.net/11336/143119
dc.identifier1432-2013
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4318771
dc.description.abstractThe soluble adenylyl cyclase (sAC) was identified in the heart as another source of cyclic AMP (cAMP). However, its cardiac physiological function is unknown. On the other hand, the cardiac Na+/HCO3 − cotransporter (NBC) promotes the cellular co-influx of HCO3 − and Na+. Since sAC activity is regulated by HCO3 −, our purpose was to investigate the potential functional relationship between NBC and sAC in the cardiomyocyte. Rat ventricular myocytes were loaded with Fura-2, Fluo-3, or BCECF to measure Ca2+ transient (Ca2+ i) by epifluorescence, Ca2+ sparks frequency (CaSF) by confocal microscopy, or intracellular pH (pHi) by epifluorescence, respectively. Sarcomere or cell shortening was measured with a video camera as an index of contractility. The NBC blocker S0859 (10 μM), the selective inhibitor of sAC KH7 (1 μM), and the PKA inhibitor H89 (0.1 μM) induced a negative inotropic effect which was associated with a decrease in Ca2+ i. Since PKA increases Ca2+ release through sarcoplasmic reticulum RyR channels, CaSF was measured as an index of RyR open probability. The generation of CaSF was prevented by KH7. Finally, we investigated the potential role of sAC activation on NBC activity. NBC-mediated recovery from acidosis was faster in the presence of KH7 or H89, suggesting that the pathway sAC-PKA is negatively regulating NBC function, consistent with a negative feedback modulation of the HCO3 − influx that activates sAC. In summary, the results demonstrated that the complex NBC-sAC-PKA plays a relevant role in Ca2+ handling and basal cardiac contractility.
dc.languageeng
dc.publisherSpringer Verlag Berlín
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00424-019-02331-x
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00424-019-02331-x
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCAMP
dc.subjectCARDIAC MYOCYTES
dc.subjectSODIUM/BICARBONATE COTRANSPORTER
dc.subjectSOLUBLE ADENYLYL CYCLASE
dc.titleThe functional association between the sodium/bicarbonate cotransporter (NBC) and the soluble adenylyl cyclase (sAC) modulates cardiac contractility
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución