dc.creatorGonzalez, Candela Rocio
dc.creatorSedó, Cristian Alvarez
dc.creatorNodar, Florencia
dc.creatorPapier, Sergio
dc.creatorVitullo, Alfredo Daniel
dc.date.accessioned2020-01-29T17:25:55Z
dc.date.accessioned2022-10-14T22:42:21Z
dc.date.available2020-01-29T17:25:55Z
dc.date.available2022-10-14T22:42:21Z
dc.date.created2020-01-29T17:25:55Z
dc.date.issued2017-11
dc.identifierGonzalez, Candela Rocio; Sedó, Cristian Alvarez; Nodar, Florencia; Papier, Sergio; Vitullo, Alfredo Daniel; Simultaneous expression analysis of deleted in azoospermia-family genes and CDC25A: their potential as a predictor for successful testicular sperm extraction; Nature Publishing Group; Asian Journal of Andrology; 19; 6; 11-2017; 715-716
dc.identifier1008-682X
dc.identifierhttp://hdl.handle.net/11336/96113
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4315255
dc.description.abstractThe Deleted in Azoospermia (DAZ) gene family consists of two autosomal genes, BOULE and DAZ-L (DAZ-like), and the DAZ gene cluster on chromosome Y. These genetic factors encode for RNA-binding proteins that are mainly expressed in germ cells and are considered essential for male fertility. Dysfunctions of these genetic factors lead to abnormal spermatogenesis and may cause male infertility that requires the use of testicular sperm extraction (TESE) for spermatozoa recovery. We analysed mRNA and protein expression of DAZ, DAZ-L, BOULE and the cell cycle regulator CDC25A, in testicular biopsies from patients with hypospermatogenesis and maturation arrest. DAZ gene family proteins were detected by immunohistochemistry in the germ cells in all biopsies. Individual expression level of DAZ, DAZ-L, BOULE and CDC25A showed no significant differences between hypospermatogenesis and maturation arrest biopsies. However, when all DAZ family members were simultaneously analyzed, we detected positive correlations between the expression level of DAZ/DAZ-L, DAZ/BOULE, DAZ-L/BOULE, DAZ-L/CDC25A and CDC25A/BOULE (p<0.05). Moreover, a significant increase in the expression of BOULE and CDC25A was detected in biopsies with successful sperm retrieval vs. biopsies with failed sperm retrieval (p<0.05). These results show that the simultaneous analysis of DAZ gene family members and CDC25A correlates with the spermatogenic damage in patients with hypospermatogenesis and maturation arrest and arises as a potential molecular predictor of sperm retrieval success by TESE.
dc.languageeng
dc.publisherNature Publishing Group
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4103/1008-682X.184993
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=6;spage=715;epage=716;aulast=Gonz%E1lez
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDAZ
dc.subjectCDC25A
dc.subjectTESTIS
dc.subjectHIPOSPERMATOGENESIS
dc.subjectMATURATION ARREST
dc.titleSimultaneous expression analysis of deleted in azoospermia-family genes and CDC25A: their potential as a predictor for successful testicular sperm extraction
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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