dc.creator | Sampedro, Maria Florencia | |
dc.creator | Izaguirre, María Fernanda | |
dc.creator | Sigot, Valeria | |
dc.date.accessioned | 2020-10-21T12:16:12Z | |
dc.date.accessioned | 2022-10-14T22:19:09Z | |
dc.date.available | 2020-10-21T12:16:12Z | |
dc.date.available | 2022-10-14T22:19:09Z | |
dc.date.created | 2020-10-21T12:16:12Z | |
dc.date.issued | 2018-12 | |
dc.identifier | Sampedro, Maria Florencia; Izaguirre, María Fernanda; Sigot, Valeria; E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]; F1000 Research Ltd; F1000Research; 7; 12-2018; 1489-1499 | |
dc.identifier | 2046-1402 | |
dc.identifier | http://hdl.handle.net/11336/116222 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4313219 | |
dc.description.abstract | Background: E-cadherin is the major adhesion receptor in epithelial adherens junctions (AJs). On established epidermis, E-cadherin performs fine-tuned cell-cell contact remodeling to maintain tissue integrity, which is characterized by modulation of cell shape, size and packing density. In zebrafish, the organization and distribution of E-cadherin in AJs during embryonic epidermis development remain scarcely described. Methods: Combining classical immunofluorescence, deconvolution microscopy and 3D-segmentation of AJs in epithelial cells, a quantitative approach was implemented to assess the spatial and temporal distribution of E-cadherin across zebrafish epidermis between 24 and 72 hpf. Results: increasing levels of E-cadh protein parallel higher cell density and the appearance of hexagonal cells in the enveloping layer (EVL) as well as the establishments of new cell-cell contacts in the epidermal basal layer (EBL), being significantly between 31 and 48 hpf. Conclusions: Increasing levels of E-cadherin in AJs correlates with extensive changes in cell morphology towards hexagonal packing during the epidermis morphogenesis. | |
dc.language | eng | |
dc.publisher | F1000 Research Ltd | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://f1000research.com/articles/7-1489/v3 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.12688/f1000research.15932.3 | |
dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 3D SEGMENTATION | |
dc.subject | DECONVOLUTION | |
dc.subject | E-CADHERIN; ADHERENS JUNCTIONS; ZEBRAFISH EPIDERMIS; ENVELOPING LAYER (EVL) | |
dc.subject | EPIDERMIS BASAL LAYER (EBL) | |
dc.title | E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved] | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |