info:eu-repo/semantics/article
Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
Registro en:
Iacoboni, Paola Anabella; Hasenauer, Flavia Carolina; Caffaro, Maria Eugenia; Gaido, Analía Beatriz; Rossetto, Cristina; et al.; Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats; Elsevier Science; Veterinary Immunology And Immunopathology; 160; 3-4; 8-2014; 230-234
0165-2427
CONICET Digital
CONICET
Autor
Iacoboni, Paola Anabella
Hasenauer, Flavia Carolina
Caffaro, Maria Eugenia
Gaido, Analía Beatriz
Rossetto, Cristina
Neumann, Roberto
Salatin, Antonio
Bertoni, Emiliano
Poli, Mario Andres
Rossetti, Carlos Alberto
Resumen
Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection. Fil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina Fil: Hasenauer, Flavia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina Fil: Caffaro, Maria Eugenia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina Fil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Yuto. Agencia de Extension Rural Oran.; Argentina Fil: Neumann, Roberto. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina Fil: Salatin, Antonio. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina Fil: Bertoni, Emiliano. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
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