dc.creator | Vázquez, Romina Laura | |
dc.creator | Daza Millone, Maria Antonieta | |
dc.creator | Pavinatto, Felippe J. | |
dc.creator | Fanani, Maria Laura | |
dc.creator | Oliveira, Osvaldo N. Jr. | |
dc.creator | Vela, Maria Elena | |
dc.creator | Maté, Sabina María | |
dc.date.accessioned | 2020-11-24T19:06:29Z | |
dc.date.accessioned | 2022-10-14T22:01:57Z | |
dc.date.available | 2020-11-24T19:06:29Z | |
dc.date.available | 2022-10-14T22:01:57Z | |
dc.date.created | 2020-11-24T19:06:29Z | |
dc.date.issued | 2019-01 | |
dc.identifier | Vázquez, Romina Laura; Daza Millone, Maria Antonieta; Pavinatto, Felippe J.; Fanani, Maria Laura; Oliveira, Osvaldo N. Jr.; et al.; Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: a PM-IRRAS study; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 173; 1-2019; 549-556 | |
dc.identifier | 0927-7765 | |
dc.identifier | http://hdl.handle.net/11336/118921 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4311648 | |
dc.description.abstract | Membrane structure is a key factor for the cell`s physiology, pathology, and therapy. Evaluating the importance of lipid species such as N-nervonoyl sphingomyelin (24:1-SM) —able to prevent phase separation— to membrane structuring remains a formidable challenge. This is the first report in which polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS) is applied to investigate the lipid-lipid interactions in 16:0 vs 24:1-SM monolayers and their mixtures with 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol (Chol) (DOPC/SM/Chol 2:1:1). From the results we inferred that the cis double bond (Δ15) in 24:1-SM molecule diminishes intermolecular H-bonding and chain packing density compared to that of 16:0-SM. In ternary mixtures containing 16:0-SM, the relative intensity of the two components of the Amide I band reflected changes in the H-bonding network due to SM-Chol interactions. In contrast, the contribution of the main components of the Amide I band in DOPC/24:1-SM/Chol remained as in 24:1-SM monolayers, with a larger contribution of the non-H-bonded component. The most interesting feature in these ternary films is that the Cdouble bondO stretching mode of DOPC appeared with an intensity similar to that of SM Amide I band in DOPC/16:0-SM/Chol monolayers (a two-phase [Lo/Le] system), whereas an extremely low intensity of the Cdouble bondO band was detected in DOPC/24:1-SM/Chol monolayers (single Le phase). This is evidence that the unsaturation in 24:1-SM affected not only the conformational properties of acyl chains but also the orientation of the chemical groups at the air/water interface. The physical properties and overall H-bonding ability conferred by 24:1-SM may have implications in cell signaling and binding of biomolecules. | |
dc.language | eng | |
dc.publisher | Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S092777651830715X | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.colsurfb.2018.10.018 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | MEMBRANE RAFTS | |
dc.subject | CHOLESTEROL-SPHINGOMYELIN INTERACTIONS | |
dc.subject | LANGMUIR MONOLAYERS | |
dc.subject | PM-IRRAS | |
dc.title | Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: a PM-IRRAS study | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |