dc.creator | Thomas, Laura | |
dc.creator | Pasquini, Laura Andrea | |
dc.date.accessioned | 2021-04-19T12:05:48Z | |
dc.date.accessioned | 2022-10-14T21:36:26Z | |
dc.date.available | 2021-04-19T12:05:48Z | |
dc.date.available | 2022-10-14T21:36:26Z | |
dc.date.created | 2021-04-19T12:05:48Z | |
dc.date.issued | 2019-10 | |
dc.identifier | Thomas, Laura; Pasquini, Laura Andrea; Galectin-3 Exerts a Pro-differentiating and Pro-myelinating Effect Within a Temporal Window Spanning Precursors and Pre-oligodendrocytes: Insights into the Mechanisms of Action; Humana Press; Molecular Neurobiology; 57; 2; 10-2019; 976-987 | |
dc.identifier | 0893-7648 | |
dc.identifier | http://hdl.handle.net/11336/130325 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4309351 | |
dc.description.abstract | Oligodendrocytes (OLG) are the cells resident in the CNS responsible for myelination. OLG undergo a succession of morphological and molecular changes along several maturational stages. Galectin-3 (Gal-3) is a 25- to 35-KDa protein belonging to the family of carbohydrate-binding galectins, which bind to glycoconjugates containing β-galactosides. Gal-3 lacks a specific receptor and its binding is thus rather unspecific, as it depends on the cellular environment and the repertoire of glycomolecules at the time when Gal-3 is present. Our previous work revealed that recombinant Gal-3 (rGal-3)–treated OLG showed accelerated differentiation, evidenced by an increase in the number of mature cells to the detriment of immature ones and accelerated actin cytoskeleton dynamics. These changes were a consequence of rGal-3 influence on Akt, Erk 1/2, and β-catenin signaling pathways. Considering this previous evidence, the aim of this study was to identify the temporal window of rGal-3 action on the OLG lineage to induce OLG maturation by using specific single pulses of rGal-3 over the different maturational stages of OLG, and to unravel its main direct targets promoting OLG differentiation by mass spectrometry analysis. Our results reveal a key temporal window spanning between OPC and pre-OLG states in which rGal-3 action promotes OLG differentiation, and identify several targets for rGal-3 binding including proteins related to the cytoskeleton, signaling pathways, metabolism and intracellular trafficking, among others. These results highlight the relevance of Gal-3 in signaling pathways regulating oligodendroglial differentiation and support a potential therapeutic role for rGal-3 in demyelinating diseases such as multiple sclerosis. | |
dc.language | eng | |
dc.publisher | Humana Press | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12035-019-01787-3 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12035-019-01787-3 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | ACTIN | |
dc.subject | GALECTIN-3 | |
dc.subject | MASS SPECTROMETRY | |
dc.subject | OLIGODENDROCYTES | |
dc.subject | SIGNAL TRANSDUCTION | |
dc.title | Galectin-3 Exerts a Pro-differentiating and Pro-myelinating Effect Within a Temporal Window Spanning Precursors and Pre-oligodendrocytes: Insights into the Mechanisms of Action | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |