Effects of maternal captopril treatment during late pregnancy on neonatal lung development in rats

dc.creatorCapelari, Diego Nicolás
dc.creatorSánchez, Susana I.
dc.creatorOrtega, Hugo H.
dc.creatorCiuffo, Gladys Maria
dc.creatorFuentes, Lucia Beatriz
dc.date2012-08
dc.identifierhttp://hdl.handle.net/11336/148321
dc.identifierCapelari, Diego Nicolás; Sánchez, Susana I.; Ortega, Hugo H.; Ciuffo, Gladys Maria; Fuentes, Lucia Beatriz; Effects of maternal captopril treatment during late pregnancy on neonatal lung development in rats; Elsevier Science; Regulatory Peptides; 177; 1-3; 8-2012; 97-106
dc.identifier0167-0115
dc.identifierCONICET Digital
dc.identifierCONICET
dc.descriptionThe renin-angiotensin system (RAS) has been implicated in pulmonary hypertension and pulmonary fibrosis. In the present study, we examined the effects of maternal exposure to captopril (2.85. mg/kg/day) during late pregnancy (G13-G21) on postnatal rat lung development. Treatment with captopril during late pregnancy caused a significant decrease in ACE activity in P0 rats. Body weight decreased at P0 (p < 0.001), P8 and P15 (p < 0.01) in captopril-treated rats. Lung weight of P0 and P8 pups was lower in treated-animals (p.< 0.05). Lungs from captopril-treated animals showed impaired alveolar formation, with enlarged distal airway spaces at P8, P15 and P30. Interalveolar wall distance measured by mean linear intercept increased in treated vs. age-matched animals at P8, P15 (p < 0.001) and P30 (p < 0.05) resembling new bronchopulmonary dysplasia. In control animals, the proliferating cell nuclear antigen (PCNA) marker was higher at P0 and then drops gradually, while in captopril-treated animals PCNA marker remains higher at all stages studied. α-Smooth muscle actin (α-SMA), a marker of fibroblast differentiation into myofibroblasts, was higher at the tips of developing secondary septa in captopril-treated lungs at P8 and P15. The increased expression of PCNA and α-SMA in treated pups suggest that beyond the effect caused by captopril, the developing lungs have the capacity to recover once the treatment was stopped. Taking together the low weight, histomorphological changes and increased expression of cellular markers caused by ACE inhibition during late pregnancy, it appears that the RAS could be an intrinsic factor involved in secondary septa formation during lung development.
dc.descriptionFil: Capelari, Diego Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
dc.descriptionFil: Sánchez, Susana I.. Universidad Nacional de San Luis; Argentina
dc.descriptionFil: Ortega, Hugo H.. Universidad Nacional del Litoral; Argentina
dc.descriptionFil: Ciuffo, Gladys Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
dc.descriptionFil: Fuentes, Lucia Beatriz. Universidad Nacional de San Luis; Argentina
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dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0167011512001590
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.regpep.2012.05.092
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectANGIOTENSIN-CONVERTING ENZYME
dc.subjectCAPTOPRIL
dc.subjectCELLULAR PROLIFERATION
dc.subjectPOSTNATAL LUNG DEVELOPMENT
dc.subjectPULMONARY DYSPLASIA
dc.subjectRENIN ANGIOTENSIN SYSTEM
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleEffects of maternal captopril treatment during late pregnancy on neonatal lung development in rats
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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