Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study

dc.creatorZhang, Meiling
dc.creatorWang, Xuebao
dc.creatorWang, Zhiyi
dc.creatorDeng, Mingjie
dc.creatorXu, Zhisheng
dc.creatorZheng, Yuancai
dc.creatorZhang, Yuan
dc.creatorHuang, Xueli
dc.date2012
dc.date2012-04-04T03:00:00Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/8447
dc.identifierhttp://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_2_6.pdf
dc.descriptionA sensitive and selective liquid chromatography-mass spectrometry (LC–MS) method for determination of esomeprazole in rabbit plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 x 150 mm, 5.0 μm) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 198 for esomeprazole and m/z 326 for midazolam (internal standard, IS). The assay was linear over the range of 10–2000 ng/mL for esomeprazole, with a lower limit of quantitation (LLOQ) of 10 ng/mL for esomeprazole. Intra- and inter-day precisions were less than 14 % and the accuracies were in the range of 89.2-112.6 % for esomeprazole. This developed method was successfully applied for the determination of esomeprazole in rabbit plasma for pharmacokinetic study.
dc.descriptionColegio de Farmacéuticos de la Provincia de Buenos Aires
dc.formatapplication/pdf
dc.format167-171
dc.languageen
dc.relationLatin American Journal of Pharmacy
dc.relationvol. 31, no. 1
dc.subjectFarmacia
dc.titleDetermination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
dc.typeArticulo
dc.typeComunicacion


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