| dc.creator | Zhou, An | |
| dc.creator | Wu, Hongfei | |
| dc.creator | Peng, Daiyin | |
| dc.creator | Li, Qinglin | |
| dc.creator | Sun, Xiaojuan | |
| dc.date | 2011 | |
| dc.date | 2011-11-11T03:00:00Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/8336 | |
| dc.identifier | http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_14.pdf | |
| dc.description | A high performance liquid chromatography photo diode array UV detection electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI/MS<sup>n</sup>) method was developed and validated for the quality evaluation of gamboge (dryed resin exuded from the stems of Garcinia hanburyi). The contents of the three bioactive constituents (gambogenic acid, R-gambogic acid and S-gambogic) were determined by using HPLC–PDA, and their chemical structures were identified by HPLC-PDA-ESI/MS<sup>n</sup>.The limits of detection and quantitation were between 0.039-0.048 μg/mL and 0.13-0.16 μg/mL. The intra- and inter- assay precisions, in terms of percent relative standard deviation, are less than 3.7 and 4.8 %, respectively . The accuracy, in terms of recovery percentage, ranged from 96.86 to 101.70 %. Good linearity (correlation coefficient > 0.9996) for each calibration curve of standards. HPLC-PDA-ESI/MS<sup>n</sup> was use to analyze caged xanthones in gamboge. A total of 16 peaks were identified or tentatively characterized. The results indicated that the method could be considered to be a simple, rapid and reliable method for the quality evaluation of gamboge. | |
| dc.description | Colegio de Farmacéuticos de la Provincia de Buenos Aires | |
| dc.format | application/pdf | |
| dc.format | 1542-1549 | |
| dc.language | en | |
| dc.relation | Latin American Journal of Pharmacy | |
| dc.relation | vol. 30, no. 8 | |
| dc.subject | Farmacia | |
| dc.title | Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup> | |
| dc.type | Articulo | |
| dc.type | Articulo | |
