Articulo
Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
Autor
Garcia, Cássia V.
Mendez, Andreas L.
Steppe, Martin
Schapoval, Elfrides E. S.
Institución
Resumen
Rabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H<sup>+</sup> /K<sup>+</sup> ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantification of RAB in pharmaceutical formulation and compare it with a capillary electrophoresis (CE) one, previously validated. The UV technique was applied using water (pH 10.0) as diluent and the determinations were made at λ = 291 nm. The method showed good linearity (r = 0.9997) in the concentration range of 6.0 to 18.0 μg ml<sup>-1</sup> .(The intra- and interday precision data demonstrated the method has good repeatability (RSD = 0.52 and 0.82, respectively . Accuracy and specificity were also evaluated and results were satisfactory. The detection and quantitation limits were 0.32 and 0.95 μg ml<sup>-1</sup> , respectively. Both methods demonstrated to be adequate for the intended purpose. Colegio de Farmacéuticos de la Provincia de Buenos Aires