Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method

dc.creatorMartins, Isarita
dc.creatorSouza, Jeferson O.
dc.creatorSanson, Ananda L.
dc.creatorVieira, Elisabeth
dc.creatorGuisti-Paiva, Alexandre
dc.date2009-06-19T03:00:00Z
dc.date2009
dc.date.accessioned2017-03-08T20:12:22Z
dc.date.available2017-03-08T20:12:22Z
dc.identifierhttp://hdl.handle.net/10915/7723
dc.identifierhttp://www.latamjpharm.org/trabajos/28/1/LAJOP_28_1_1_6_O9LZ159788.pdf
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/7723
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/429790
dc.descriptionA reproducible and selective method for the simultaneous determination of cyclophosphamide (CP) and ifosfamide (IF) in plasma has been developed and validated using isocratic elution. The assay is performed by HPLC-UV, with a C18 column (5μm, 150 x 4 mm) and detection in 195 nm. The mobile phase was constituted by phosphate buffer 10 mM pH 6.0: acetonitrile (77.25:22.75), with a flow of 1 mL/min. SPE was used for sample clean-up in a range from 3 (LOQ) to 540 μM. The repeatability coefficients of variation (CV) ranged from 0.5 to 7.8% and the intermediate precision CVs varied from 0.9 to 5.7%. Extraction efficacy and accuracy varied from 94 to 115%. The simple method may permit the determination of cyclophosphamide and ifosfamide in plasma, simultaneously, to pharmacokinetics and bioequivalence studies.
dc.languageen
dc.relationLatin American Journal of Pharmacy
dc.relationvol. 28, no. 1
dc.subjectantineoplastic drugs; HPLC; cyclophosphamide; Ifosfamide; simultaneous determination
dc.subjectFarmacia
dc.subjectProductos químicos y drogas
dc.subjectEvaluación de drogas
dc.subjectToxicología
dc.subjectPreparaciones farmacéuticas
dc.titleSimultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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