Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes

dc.creatorBrovedan, Marco Alcides
dc.creatorMarchiaro, Patricia M.
dc.creatorDíaz, María S.
dc.creatorFaccone, Diego
dc.creatorCorso, Alejandra
dc.creatorPasteran, Fernando
dc.creatorViale, Alejandro M.
dc.creatorLimansky, Adriana S.
dc.date2022-05-16T14:57:50Z
dc.date2022-05-16T14:57:50Z
dc.date2021-11-06
dc.date2022-05-16T14:57:50Z
dc.date2022-05-16T14:57:50Z
dc.date2021-11-06
dc.identifier1567-1348
dc.identifierhttp://hdl.handle.net/2133/23644
dc.identifierhttp://hdl.handle.net/2133/23644
dc.descriptionThe Pseudomonas putida group (P. putida G) is composed of at least 21 species associated with a wide range of environments, including the clinical setting. Here, we characterized 13 carbapenem-resistant P. putida G clinical isolates bearing class 1 integrons/transposons (class 1 In/Tn) carrying blaVIM-2 metallo-β-lactamase gene cassettes obtained from hospitals of Argentina. Multilocus sequencing (MLSA) and phylogenetic analyses based on 16S rDNA, gyrB and rpoD sequences distinguished 7 species among them. blaVIM-2 was found in three different cassette arrays: In41 (blaVIM-2-aacA4), In899 (only blaVIM-2), and In528 (dfrB1-aacA4-blaVIM-2). In41 and In899 were associated with complete tniABQC transposition modules and IRi/IRt boundaries characteristic of the Tn5053/Tn402 transposons, which were designated Tn6335 and Tn6336, respectively. The class 1 In/Tn element carrying In528, however, exhibited a defective tni module bearing only the tniC (transposase) gene, associated with a complete IS6100 bounded with two oppositely-oriented IRt end regions. In some P. putida G isolates including P. asiatica, P. juntendi, P. putida G/II, and P. putida G/V, Tn6335/Tn6336 were carried by pLD209-type conjugative plasmids capable of self-mobilization to P. aeruginosa or Escherichia coli. In other isolates of P. asiatica, P. putida G/II, and P. monteiliieilii, however, these blaVIM-2-containing class 1 In/Tn elements were found inserted into the res regions preceding the tnpR (resolvase) gene of particular Tn21 subgroup members of Tn3 transposons. The overall results reinforce the notion of P. putida G members as blaVIM-2 reservoirs, and shed light on the mechanisms of dissemination of carbapenem resistance genes to other pathogenic bacteria in the clinical setting.
dc.descriptionFil: Brovedan, Marco Alcides. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Marchiaro, Patricia M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Díaz, María S. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Faccone, Diego. ANLIS Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobiano; Argentina.
dc.descriptionFil: Corso, Alejandra. ANLIS Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobiano; Argentina.
dc.descriptionFil: Pasteran, Fernando. ANLIS Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobiano; Argentina.
dc.descriptionFil: Viale, Alejandro M.Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Limansky, Adriana S. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier B.V.
dc.relationhttps://www.sciencedirect.com/science/article/pii/S1567134821004317?via%3Dihub#s0100
dc.relationhttps://doi.org/10.1016/j.meegid.2021.105131
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsElsevier B. V.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rightsopenAccess
dc.subjectPseudomonas putida group
dc.subjectCarbapenem resistance plasmids
dc.subjectTn402-like class 1 integrons
dc.subjectVIM-2 metallo-β-lactamase reservoirs
dc.subjectMobile genetic elements
dc.subjectTn21
dc.titlePseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
dc.typepublishedVersion
dc.typearticle
dc.typeartículo


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