| dc.creator | Steeman, Tomás José | |
| dc.creator | Rubiolo, Juan Andrés | |
| dc.creator | Sánchez, Laura E. | |
| dc.creator | Calcaterra, Nora B. | |
| dc.creator | Weiner, Andrea María Julia | |
| dc.date | 2022-03-28T20:02:35Z | |
| dc.date | 2022-03-28T20:02:35Z | |
| dc.date | 2021 | |
| dc.date | 2022-03-28T20:02:35Z | |
| dc.date | 2022-03-28T20:02:35Z | |
| dc.date | 2021 | |
| dc.identifier | 2073-4425 | |
| dc.identifier | http://hdl.handle.net/2133/23272 | |
| dc.identifier | http://hdl.handle.net/2133/23272 | |
| dc.description | The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have been extensively studied to date. Although several publications suggest a common role for microRNA-145 (miR-145) in molecular reprogramming for cell cycle regulation and/or cellular differ entiation, little is known about its role during in vivo cranial neural crest development. By modifying miR-145 levels in zebrafish embryos, abnormal craniofacial development and aberrant pigmentation phenotypes were detected. By whole-mount in situ hybridization, changes in expression patterns of col2a1a and Sry-related HMG box (Sox) transcription factors sox9a and sox9b were observed in overexpressed miR-145 embryos. In agreement, zebrafish sox9b expression was downregulated by miR-145 overexpression. In silico and in vivo analysis of the sox9b 3 0UTR revealed a conserved potential miR-145 binding site likely involved in its post-transcriptional regulation. Based on these findings, we speculate that miR-145 participates in the gene regulatory network governing zebrafish chondrocyte differentiation by controlling sox9b expression. | |
| dc.description | Fil: Steeman, Tomás José. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Argentina. | |
| dc.description | Fil: Rubiolo, Juan Andrés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Argentina. | |
| dc.description | Fil: Sánchez, Laura E. Universidade de Santiago de Compostela. Facultad de Veterinaria. Departamento de Zoología Genética y Antropología Física. | |
| dc.description | Fil: Calcaterra, Nora B. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Argentina. | |
| dc.description | Fil: Weiner, Andrea María Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Argentina. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | MDPI | |
| dc.relation | https://www.mdpi.com/2073-4425/12/7/1023 | |
| dc.relation | https://doi.org/10.3390/genes12071023 | |
| dc.rights | http://creativecommons.org/licenses/by/2.5/ar/ | |
| dc.rights | Steeman, Tomás José | |
| dc.rights | Rubiolo, Juan Andrés | |
| dc.rights | Sánchez, Laura E. | |
| dc.rights | Calcaterra, Nora B. | |
| dc.rights | Weiner, Andrea María Julia. | |
| dc.rights | Atribución 4.0 Internacional (CC BY 4.0) | |
| dc.rights | openAccess | |
| dc.subject | MicroRNA | |
| dc.subject | Neural Crest | |
| dc.subject | Gene Regulatory Network | |
| dc.subject | Embryonic Developmen | |
| dc.title | Conservation of zebrafish microrna-145 and its role during neural crest cell development | |
| dc.type | publishedVersion | |
| dc.type | article | |
| dc.type | artículo | |
