Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco

dc.creatorNejamkin, Andrés
dc.creatorForesi, Noelia
dc.creatorMayta, Martín L.
dc.creatorLodeyro, Anabella F.
dc.creatorDel Castello, Fiorella
dc.creatorCorrea-Aragunde, Natalia
dc.creatorCarrillo, Néstor
dc.creatorLamattina, Lorenzo
dc.date2020-08-27T12:11:57Z
dc.date2020-08-27T12:11:57Z
dc.date2020-03-20
dc.date2020-08-27T12:11:57Z
dc.date2020-08-27T12:11:57Z
dc.date2020-03-20
dc.identifier1664-462X
dc.identifierhttp://hdl.handle.net/2133/18754
dc.identifierhttp://hdl.handle.net/2133/18754
dc.descriptionNitric oxide (NO) is a messenger molecule widespread studied in plant physiology. Latter evidence supports the lack of a NO-producing system involving a NO synthase (NOS) activity in higher plants. However, a NOS gene from the unicellular marine alga Ostreococcus tauri (OtNOS) was characterized in recent years. OtNOS is a genuine NOS, with similar spectroscopic fingerprints to mammalian NOSs and high NO producing capacity. We are interested in investigating whether OtNOS activity alters nitrogen metabolism and nitrogen availability, thus improving growth promotion conditions in tobacco. Tobacco plants were transformed with OtNOS under the constitutive CaMV 35S promoter. Transgenic tobacco plants expressing OtNOS accumulated higher NO levels compared to siblings transformed with the empty vector, and displayed accelerated growth in different media containing sufficient nitrogen availability. Under conditions of nitrogen scarcity, the growth promoting effect of the OtNOS expression is diluted in terms of total leaf area, protein content and seed production. It is proposed that OtNOS might possess a plant growth promoting effect through facilitating N remobilization and nitrate assimilation with potential to improve crop plants performance.
dc.descriptionFil: Nejamkin, Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina.
dc.descriptionFil: Foresi, Noelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina.
dc.descriptionFil: Mayta, Martín L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Lodeyro, Anabella F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Del Castello, Fiorella. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina.
dc.descriptionFil: Correa-Aragunde, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina.
dc.descriptionFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Lamattina, Lorenzo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.relationhttps://www.frontiersin.org/articles/10.3389/fpls.2020.00312/full#ack1
dc.relationhttps://doi.org/10.3389/fpls.2020.00312
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.rightsNejamkin, Andrés
dc.rightsForesi, Noelia
dc.rightsMayta, Martín L.
dc.rightsLodeyro, Anabella F.
dc.rightsDel Castello, Fiorella
dc.rightsCorrea-Aragunde, Natalia
dc.rightsCarrillo, Néstor
dc.rightsLamattina, Lorenzo
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rightsopenAccess
dc.subjectNitric oxide
dc.subjectNitric oxide synthase
dc.subjectNicotiana tabacum
dc.subjectNitrogen
dc.subjectPlant growth
dc.subjectSeed production
dc.subjectTobacco
dc.titleNitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
dc.typearticle
dc.typeartículo
dc.typepublishedVersion


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