Biosynthesis of heme O in intraerythrocytic stages of Plasmodium falciparum and potential inhibitors of this pathway

dc.creatorSimão-Gurge, Raquel M.
dc.creatorWunderlich, Gerhard
dc.creatorCricco, Julia Alejandra
dc.creatorGalindo Cubillos, Eliana F.
dc.creatorDomenech-Carbó, Antonio
dc.creatorCebrián-Torrejón, Gerardo
dc.creatorAlmeida, Fernando G.
dc.creatorCirulli, Brenda Analía
dc.creatorKatzin, Alejandro M.
dc.date2020-06-08T19:36:02Z
dc.date2020-06-08T19:36:02Z
dc.date2019-12-17
dc.date2020-06-08T19:36:02Z
dc.date2020-06-08T19:36:02Z
dc.date2019-12-17
dc.date.accessioned2022-10-14T19:57:34Z
dc.date.available2022-10-14T19:57:34Z
dc.identifier2045-2322
dc.identifierhttp://hdl.handle.net/2133/18326
dc.identifierhttp://hdl.handle.net/2133/18326
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4292158
dc.descriptionA number of antimalarial drugs interfere with the electron transport chain and heme-related reactions; however, the biosynthesis of heme derivatives in Plasmodium parasites has not been fully elucidated. Here, we characterized the steps that lead to the farnesylation of heme. After the identification of a gene encoding heme O synthase, we identified heme O synthesis in blood stage parasites through the incorporation of radioactive precursors. The presence of heme O synthesis in intraerythrocytic stages of Plasmodium falciparum was confirmed by mass spectrometry. Inabenfide and uniconazole–P appeared to interfere in heme synthesis, accordingly, parasite growth was also affected by the addition of these drugs. We conclude that heme O synthesis occurs in blood stage-P. falciparum and this pathway could be a potential target for antimalarial drugs.
dc.descriptionFil: Simão-Gurge, Raquel M. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brazil
dc.descriptionFil: Wunderlich, Gerhard. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brazil
dc.descriptionFil: Cricco, Julia Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Galindo Cubillos, Eliana F. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brazil
dc.descriptionFil: Domenech-Carbó, Antonio. University of Valencia. Faculty of Chemistry. Department of Analytical Chemistry; Spain
dc.descriptionFil: Cebrián-Torrejón, Gerardo. University of Valencia. Faculty of Chemistry. Department of Analytical Chemistry; Spain
dc.descriptionFil: Cebrián-Torrejón, Gerardo. Université des Antilles. Laboratoire COVACHIM-M2E EA 3592; France
dc.descriptionFil: Almeida, Fernando G. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brazil
dc.descriptionFil: Cirulli, Brenda Analía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.descriptionFil: Katzin, Alejandro M. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brazil
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Nature
dc.relationhttps://doi.org/10.1038/s41598-019-55506-y
dc.relationhttps://www.nature.com/articles/s41598-019-55506-y
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.rightsSimão-Gurge, Raquel M.
dc.rightsWunderlich, Gerhard
dc.rightsCricco, Julia Alejandra
dc.rightsGalindo Cubillos, Eliana F.
dc.rightsDomenech-Carbó, Antonio
dc.rightsCebrián-Torrejón, Gerardo
dc.rightsAlmeida, Fernando G.
dc.rightsCirulli, Brenda Analía
dc.rightsKatzin, Alejandro M.
dc.rightsUniversidad Nacional de Rosario
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rightsopenAccess
dc.subjectAntimalarial drugs
dc.subjectPlasmodium parasites
dc.subjectHeme O synthesis
dc.subjectPlasmodium Falciparum
dc.subjectInhibitors
dc.titleBiosynthesis of heme O in intraerythrocytic stages of Plasmodium falciparum and potential inhibitors of this pathway
dc.typearticle
dc.typeartículo
dc.typepublishedVersion


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