Artículos de revistas
Ácido 1-amino-3-metilciclobutano carboxílico en semillas de Ateleia glazioviana Baillon (Leguminosae)
1-Amino-3-methylcyclobutane carboxylic acid in seeds of Ateleia glazioviana Baillon (Leguminosae)
Autor
Marona, Hérida Regina Nunes
González Ortega, George
Schenkel, Eloir Paulo
Huet, Jean
Institución
Resumen
Un nuevo aminoácido del tipo no proteico, el ácido 1-amino-3-metilciclobutano carboxílico, fue aislado de las semillas de Ateleia glazioviana Baill. extraídas con etanol 50%. El extracto alcohólico, después de ser purificado por extracción sucesiva con diclorometano, acetato de etilo y n-butanol, fue fraccionado en una columna con resina de intercambio iónico. La separación final fue realizada por CC con sílica gel, con un rendimiento final de 0,02%. La estructura química fue establecida a través de IR, EIMS de alta resolución y <sup>13</sup>C y <sup>-1</sup>H-RMN. The structure of a novel nonprotein amino acid isolated from the seeds of Ateleia glazioviuna Baillon was established as 1-amino-3-methylcyclobutanec arboxylic acid on basis of spectroscopic evidentes. For the isolation, the ethanol-water extract was successively extracted with dichloromethane, ethyl acetate and n-butano. Thereafter, the amino acids were separated from the remaining water phase using a cation-exchange resin column and further submited to column chromatography on silica gel to yield 80 mg (0,02%) of the new amino acid.
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