dc.creatorPáez, María Angélica Reyes
dc.creatorTovar, Natalia Johana Rojas
dc.creatorMendoza-Sánchez, Geovanny
dc.creatorMuñoz, Marcela Fernández
dc.creatorMolina, Boris Julián Sepúlveda
dc.creatorTorres, Andrés Felipe Santander
dc.creatorGutíerrez, Jesús Alfredo Berdugo
dc.creatorBuitrago, Jhon Didier Ruiz
dc.date2021-01-01T08:00:00Z
dc.date.accessioned2022-10-13T15:55:49Z
dc.date.available2022-10-13T15:55:49Z
dc.identifierhttps://ciencia.lasalle.edu.co/scopus_unisalle/913
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4189682
dc.descriptionSince the 19th century, the presentation of bovines with disproportionate muscle development have been associated with mutations that inhibit the action of the myostatin gene, it is referred to as double muscle mutation, which is common in some European Bos taurus breeds but it is not reported in buffaloes Bubalus bubalis. This study aims to evaluate if the phenotype observed in 6 young buffaloes with disproportionate muscle development has the same myostatin mutation reported in cattle. DNA was obtained from the blood of the animals of the Murrah breed. First, second and third exon was amplified end point PCR; the fragments were sequenced using capillary electrophoresis. Holstein cattle (Bos taurus) was used As control for normal phenotype. The results obtained from the comparison of the sequence of the myostatin gene show that the observed double-muscled phenotype did not show differences from normal controls. Interspecific variation was demonstrated by comparing exons two and three of the gene, finding 12 variations between the Bos taurus and Bubalus bubalis species in the evaluated fragments. It is necessary to study physiology, and the animals to explain the phenotype observed in buffaloes.
dc.sourceJournal of Buffalo Science
dc.source1
dc.subjectBubalus bubalis
dc.subjectDouble muscle
dc.subjectMuscle hyperplasia
dc.subjectMutation
dc.subjectMyostatin
dc.titleSequencing and analysis of the myostatin gene (GDF-8) in bubalus bubalis young animals to determine the existence of possible mutations expressed in double musculature phenotype
dc.typeArticle


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