Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae).

Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae).

dc.creatorNADIA GUADALUPE SANCHEZ COELLO
dc.creatorMAURICIO LUNA RODRIGUEZ
dc.creatorSANTIAGO MARIO VAZQUEZ TORRES
dc.creatorLAZARO RAFAEL SANCHEZ VELASQUEZ
dc.creatorNANCY SANTANA BUZZY
dc.creatorPABLO OCTAVIO AGUILAR
dc.creatorLOURDES GEORGINA IGLESIAS ANDREU
dc.date2012
dc.date.accessioned2022-10-12T20:12:44Z
dc.date.available2022-10-12T20:12:44Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1264
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4130327
dc.descriptionMost of the cycads contain high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA extraction, causing erroneous or no PCR products. The optimization of DNA isolation, employing inter-simple sequence repeats (ISSRs) primers were investigated in Ceratozamia mexicana Brongn., an endangered Mexican cycad. The DNA obtained from fresh-leaf tissues with a modified cetyltrimethylammonium bromide buffer protocol gave a good quality of DNA with no colored pigments and contaminants. The main modification to the CTAB-based DNA extraction protocol was the one hour leaf tissue soaking pre-treatment with a 0.7 M NaCl solution, to facilitate the cell lysis. The DNA extracted was successfully amplified by PCR using six arbitrary ISSR primers. Reproducible amplifiable products were observed in all PCR reactions. Our results show a significant improvement in the DNA quality obtained using low primer concentration (25 pM). 23 strong bands were detected, 9 of which were polymorphic. The results indicated that the optimized protocol for DNA isolation and PCR system is suitable for further work in this specie. This work is the first DNA extraction and ISSR protocols reported for this ornamental and endangered species.
dc.formatapplication/pdf
dc.languagespa
dc.relationinfo:eu-repo/semantics/datasetDOI/http://dx.doi.org/10.5154/r.rchscfa.2011.03.024
dc.relationcitation:Sánchez-Coello, N. G., Luna-Rodríguez, M., Vázquez-Torres, S. M., Sánchez-Velásquez, L. R., Santana-Buzzy, N., Octavio-Aguilar, P., & Iglesias-Andreu, L. G. (2012). Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae). Revista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceRevista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133, 2012.
dc.subjectinfo:eu-repo/classification/Autores/CERATOZAMIA MEXICANA
dc.subjectinfo:eu-repo/classification/Autores/CICADAS
dc.subjectinfo:eu-repo/classification/Autores/EXTRACCION DE ADN
dc.subjectinfo:eu-repo/classification/Autores/ESPECIES EN PELIGRO
dc.subjectinfo:eu-repo/classification/Autores/ISSR
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleOptimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae).
dc.titleOptimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae).
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución