dc.contributor | Vitorino, Fernanda Regina Casagrande Giachini | |
dc.contributor | Lima, Victor Vitorino | |
dc.contributor | http://lattes.cnpq.br/7503102443670435 | |
dc.contributor | http://lattes.cnpq.br/3100345884689140 | |
dc.contributor | Vitorino, Fernanda Regina Casagrande Giachini | |
dc.contributor | 712.962.121-49 | |
dc.contributor | http://lattes.cnpq.br/3100345884689140 | |
dc.contributor | Mimura, Kallyne Kioko Oliveira | |
dc.contributor | 339.307.118-18 | |
dc.contributor | http://lattes.cnpq.br/8017096776183586 | |
dc.contributor | 712.962.121-49 | |
dc.contributor | 005.356.961-08 | |
dc.date.accessioned | 2018-04-05 | |
dc.date.accessioned | 2021-05-13T19:44:51Z | |
dc.date.accessioned | 2022-10-12T18:18:08Z | |
dc.date.available | 2018-04-05 | |
dc.date.available | 2021-05-13T19:44:51Z | |
dc.date.available | 2022-10-12T18:18:08Z | |
dc.date.created | 2018-04-05 | |
dc.date.created | 2021-05-13T19:44:51Z | |
dc.date.issued | 2018-03-01 | |
dc.identifier | BRESSAN, Alecsander Fabricio Moreira. Interleucina-10 inibe a quinase regulada por sinal extracelular 1 e 2 (ERK 1/2) em aorta de camundongos hipertensos pela infusão de angiotensina II. 2018. 61 f. Dissertação (Mestrado em Imunologia e Parasitologia Básicas e Aplicadas) - Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2018. | |
dc.identifier | http://ri.ufmt.br/handle/1/2508 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4093959 | |
dc.description.abstract | The activation of extracellular signal-regulated kinase 1 and 2 (ERK 1/2) pathway promotes
increased vascular contractility in angiotensin II (Ang II)-induced hypertensive mice.
Interleukin-10 (IL-10) is an immuno-regulatory cytokine with the ability to prevent vascular
hypercontractility during hypertension. Hypothesized that IL-10 may act negatively on the
ERK 1/2 pathway in vascular tissue during hypertension.The aim was to evaluate if IL-10
modulates vascular ERK 1/2 activation during Ang II-induced hypertension. Wild-type (WT)
or IL-10 knockouts (IL-10-/-) mice received Ang II infusion (90 ηg/min, for 14 days) or
vehicle (saline), via osmotic mini-pumps, whereas another WT group were infused with
exogenous IL-10 (0.5 ηg/min for 14 days) simultaneously, or not, with Ang II. The mean
arterial pressure was measured by direct measurement technique. The plasma concentration of
IL-10 was quantified by the ELISA technique. Aortic rings were mounted in a myograph and
concentration-response curve to phenylephrine were evaluated, in the presence or absence of
ERK 1/2 inhibitor (PD98059, 10 μM, 40 minutes). Protein expression of vascular ERK 1/2
was determined by western blotting. Ang II infusion reduced plasma IL-10 levels, while
exogenous IL-10 infusion restored plasma levels of this cytokine in Ang II-infused mice. Ang
II infusion increased the maximal contraction response [milinewton (mN) corrected by% KCL] in both WT mice [218.9 ± 10.45 (Ang II) vs. 160.1 ±
7.78 (vehicle)]; and IL-10-/-[244.7 ± 5.50 (Ang II) vs. 202.1 ± 9.2 (vehicle)]. The
concomitant infusion of IL-10 and Ang II prevented vascular hypercontractility in WT mice
compared to the group infused only with Ang II (171.1 ± 5.8 vs. 218.9 ± 10.45 respectively).
Inhibition of the ERK 1/2 pathway ameliorated Ang II induced hypercontractility. Activation
1/2 vascular ERK was amplified in arteries of infused Ang II IL-10-/- mice. The infusion of
exogenous IL-10 was able to prevent vascular expression of total and phosphorylated ERK
1/2, induced by Ang II. Our results suggest that IL-10 negatively modulates vascular
activation of ERK 1/2, preventing the actions of Ang II on vascular function during
hypertension. | |
dc.publisher | Universidade Federal de Mato Grosso | |
dc.publisher | Brasil | |
dc.publisher | Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia | |
dc.publisher | UFMT CUA - Araguaia | |
dc.publisher | Programa de Pós-Graduação em Imunologia e Parasitologia Básicas e Aplicadas | |
dc.rights | Acesso Aberto | |
dc.title | Interleucina-10 inibe a quinase regulada por sinal extracelular 1 e 2 (ERK 1/2) em aorta de camundongos hipertensos pela infusão de angiotensina II | |
dc.type | Tesis | |