Efeito do composto “mais vida” adsorvido a microesfera de polietilenoglicol na sobrevivência de linhagens de células de câncer de (MCF-7).

Abstract

Breast cancer is one of the most frequent diseases among women, and now the search for more effective treatments is still a challenge. In this sense the development of drugs and / or herbal compounds has increased in recent years and the combination of active ingredients modified release systems have been used to enhance or stimulate immune responses. Medicinal plants have always been objects of study in an attempt to find new sources of active ingredients. Among them stands out the Herbal Mixture compound having modulatory activity can activate the immune system. This compound adsorbed to the microsphere polyethylene glycol was able to increase the functional activity of human phagocytes is important in bacterial infections, but its effects on tumor cells has not yet been elucidated. This work aimed to evaluate the effects of the compound Herbal Mixture adsorbed polyethylene glycol microsphere in tumor breast cell lines (MCF-7) in co-culture with mononuclear cells blood. Were analyzed in the presence of compound Herbal Mixture adsorbed microsphere polyethylene glycol blood viscosity, as well as the inhibition of proliferation, apoptosis, cell viability, release of intracellular calcium mononuclear cells in coculture with mammary adenocarcinoma cells (MCF-7). It was found that compound Herbal Mixture does not change the viscosity of normal blood. When we assessed cell viability in the presence of compound Herbal Mixture polyethylene glycol adsorbed microsphere was observed that the viability rate after 24 hours was 95.55% for mononuclear cells and 40.19% for MCF-7 cells. An increase of apoptosis and intracellular calcium in MCF-7 cells in the presence of compound Herbal Mixture. The co cell culture mononuclear and MCF-7 in presence of compound Herbal Mixture polyethylene glycol adsorbed to

reduced cell viability, increased apoptosis rates and release of intracellular calcium. These data suggest that compound Herbal Mixture absorbed microsphere polyethylene glycol can act as functional material with antitumor activity against MCF-7 cells.