Estudos de resíduos agroindustriais brasileiros como fonte de compostos de alto valor agregado: métodos verdes para a extração de flavonoides derivados de resíduos de laranja

Abstract

Currently, there is a need to increase the research aimed at the recovery of agro-industrial and forest residues worldwide. The food industry has produced an enormous amount of waste globally, among them the citrus waste that has a high potential to added value compounds, such as soluble sugars, cellulose and hemicellulose, pectin, d-limonene, ethanol and flavonoids. In this context, the aim of this project is to develop green methodologies applied to the extraction and analysis of natural bioactive compounds, such as flavonoids from orange waste derived of conventional and organic farming process. The sample preparation was carried out based on green techniques (microwave assisted extraction, ultrasound, conventional extraction with homogenizer aid), and a quantitative and qualitative determination through chromatography‘s techniques coupled to UV-Vis detector. In order to understand the behavior of the bioactive compounds of this matrix, four flavonoids (hesperidin, naringenin, hesperetin and nobiletin) were studied. The results showed that these compounds have a similar behavior to the variables tested, such as solvent extractor, temperature, time, among others, however they present differences when those are applied together in the different methods.