Involvement of GDF-9, leptin, and IGF1 receptors associated with adipose tissue transplantation on fertility restoration in obese anovulatory mice

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorSao Leopoldo Mand
dc.creatorPereira Júnior, Melquíades [UNIFESP]
dc.creatorVidotti, Daniela Berguio [UNIFESP]
dc.creatorBorra, Ricardo Carneiro
dc.creatorSimoes, Manuel de Jesus [UNIFESP]
dc.creatorSilva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.creatorHaidar, Mauro Abi [UNIFESP]
dc.date.accessioned2016-01-24T14:17:19Z
dc.date.accessioned2022-10-07T21:33:47Z
dc.date.available2016-01-24T14:17:19Z
dc.date.available2022-10-07T21:33:47Z
dc.date.created2016-01-24T14:17:19Z
dc.date.issued2011-10-01
dc.identifierGynecological Endocrinology. New York: Informa Healthcare, v. 27, n. 10, p. 759-766, 2011.
dc.identifier0951-3590
dc.identifierhttp://repositorio.unifesp.br/handle/11600/34135
dc.identifier10.3109/09513590.2010.534330
dc.identifierWOS:000295617600006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4030111
dc.description.abstractThe aim was to analyze the effect of adipose tissue transplantation on growth differentiation factor-9 (GDF-9), insulin growth factor 1 receptor (IGF1R), and leptin receptor (LEPR) protein expression in ovaries of obese anovulatory mice. Leptin-deficient female (ob/ob) and wild-type mice were divided into untreated ob/ob mice and gonadal white adipose tissue transplanted ob/ob mice, with evaluation after 7, 15, and 45 days and compared to control wild-type mice. the corporal weight and glycemia levels increased in the obese group concomitant with polymicrocyst formation and abundant estrone, mimicking anovulatory disease. in the treated group after 45 days, glycemia, weight, ovarian size, and number of follicles were decreased and corpora lutea were decreased. the analysis of GDF-9 revealed that, whereas control ovaries presented follicular localization, the obese ovary lacked this protein. On the other hand, obese ovaries showed elevated expression of IGF1R that was normalized after the transplantation. Finally, LEPR was reduced in obese ovaries, and adipose tissue transplantation was efficient in returning it to normal levels. in conclusion, the adipose tissue transplantation, especially after 45 days, seems to stimulate ovulation, supported by the fact that several proteins involved in ovulation returned to basal levels.
dc.languageeng
dc.publisherInforma Healthcare
dc.relationGynecological Endocrinology
dc.rightshttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.rightsAcesso restrito
dc.subjectPolymicrocystic ovaries
dc.subjectanovulation
dc.subjectadipose tissue transplant
dc.subjectobesity
dc.subjectGDF-9
dc.subjectIGF1R
dc.subjectLEPR
dc.titleInvolvement of GDF-9, leptin, and IGF1 receptors associated with adipose tissue transplantation on fertility restoration in obese anovulatory mice
dc.typeArtigo


Este ítem pertenece a la siguiente institución