Frequency of Toxoplasma gondii in the retina in eye banks in Brazil

dc.creatorCosta, Deise F. [UNIFESP]
dc.creatorNascimento, Heloisa [UNIFESP]
dc.creatorSutili, Aline [UNIFESP]
dc.creatorNobrega, Fernando A. J.
dc.creatorFowler, Flavio [UNIFESP]
dc.creatorNobrega, Mario Junqueira
dc.creatorGarrido, Cristina
dc.creatorDias, Janaina de Oliveira
dc.creatorAdan, Consuelo B. D.
dc.creatorRizzo, Luiz Vicente
dc.creatorSilveira, Claudio
dc.creatorBelfort, Rubens, Jr. [UNIFESP]
dc.creatorCommodaro, Alessandra G. [UNIFESP]
dc.date.accessioned2020-06-26T16:30:32Z
dc.date.accessioned2022-10-07T21:33:25Z
dc.date.available2020-06-26T16:30:32Z
dc.date.available2022-10-07T21:33:25Z
dc.date.created2020-06-26T16:30:32Z
dc.date.issued2017
dc.identifierParasitology Research. New York, v. 116, n. 7, p. 2031-2033, 2017.
dc.identifier0932-0113
dc.identifierhttps://repositorio.unifesp.br/handle/11600/53609
dc.identifier10.1007/s00436-017-5474-2
dc.identifierWOS:000403940600031
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4030062
dc.description.abstractOcular toxoplasmosis is the main cause of posterior uveitis worldwide frequently leading to vision loss. In Brazil, the seroprevalence of Toxoplasma gondii infection ranges from 50 to 80% depending of the region studied. The frequency of toxoplasmic retinal scar may reach 18% of the adults in the South of Brazil. Our goal was to determine the frequency of T. gondii DNA in retinas from eye banks from different regions in Brazil. A total of 162 eyes were obtained from eye banks in Manaus (n = 60), Sao Paulo (n = 60), Chapeco (n = 26), and Joinville (n = 16). The retinas were macroscopically analyzed and collected for DNA extraction. Real-time PCR (qPCR) was performed using the T. gondii B1 marker. By qPCR, a higher frequency of T. gondii DNA in the retinas from the eye bank of Joinville (25%) was found when compared to Manaus (5%). The retinas from Sao Paulo and Chapeco were qPCR negative. Clinical examination determined the retina lesions to be compatible with toxoplasmosis in the following frequencies: Joinville (62.5%), Manaus (10%), Sao Paulo (6.7%), and Chapeco (15.4%).
dc.languageeng
dc.publisherSpringer
dc.relationParasitology Research
dc.rightsACESSO RESTRITO
dc.subjectEye bank
dc.subjectBrazil
dc.subjectToxoplasma gondii
dc.subjectReal-time PCR
dc.titleFrequency of Toxoplasma gondii in the retina in eye banks in Brazil
dc.typeArtigo


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