Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

dc.creatorBertholim, Luciana
dc.creatorZelanis, Andre [UNIFESP]
dc.creatorOliveira, Ana K.
dc.creatorSerrano, Solange M. T.
dc.date.accessioned2020-07-22T13:23:00Z
dc.date.accessioned2022-10-07T21:33:22Z
dc.date.available2020-07-22T13:23:00Z
dc.date.available2022-10-07T21:33:22Z
dc.date.created2020-07-22T13:23:00Z
dc.date.issued2016
dc.identifierAmino Acids. Wien, v. 48, n. 5, p. 1331-1335, 2016.
dc.identifier0939-4451
dc.identifierhttps://repositorio.unifesp.br/handle/11600/55982
dc.identifier10.1007/s00726-016-2218-z
dc.identifierWOS:000374375300019
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4030053
dc.description.abstractThe Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6' sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1' position, followed by hydrophobic residues in P2'. PICS confirmed existing data on prime site specificity of SVMPs.
dc.languageeng
dc.publisherSpringer Wien
dc.relationAmino Acids
dc.rightsAcesso restrito
dc.subjectSnake venom
dc.subjectMetalloproteinase
dc.subjectProteomic identification of protease cleavage sites
dc.subjectPeptide bond specificity
dc.subjectProteome derived peptide library
dc.subjectHF3
dc.titleProteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase
dc.typeArtigo


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