Brabykinin B1 Receptor Antagonism Is Beneficial in Renal Ischemia-Reperfusion Injury

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorWang, Pamella H. M. [UNIFESP]
dc.creatorCampanholle, Gabriela
dc.creatorCenedeze, Marcos A. [UNIFESP]
dc.creatorFeitoza, Carla Q. [UNIFESP]
dc.creatorGoncalves, Giselle M. [UNIFESP]
dc.creatorLandgraf, Richardt G.
dc.creatorJancar, Sonia
dc.creatorPesquero, Joao B. [UNIFESP]
dc.creatorPacheco-Silva, Alvaro [UNIFESP]
dc.creatorCâmara, Niels Olsen Saraiva [UNIFESP]
dc.date.accessioned2016-01-24T13:51:38Z
dc.date.accessioned2022-10-07T21:32:02Z
dc.date.available2016-01-24T13:51:38Z
dc.date.available2022-10-07T21:32:02Z
dc.date.created2016-01-24T13:51:38Z
dc.date.issued2008-08-25
dc.identifierPlos One. San Francisco: Public Library Science, v. 3, n. 8, 9 p., 2008.
dc.identifier1932-6203
dc.identifierhttp://repositorio.unifesp.br/handle/11600/30856
dc.identifierWOS000264429000003.pdf
dc.identifier10.1371/journal.pone.0003050
dc.identifierWOS:000264429000003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4029887
dc.description.abstractPreviously we have demonstrated that bradykinin B1 receptor deficient mice (B1KO) were protected against renal ischemia and reperfusion injury (IRI). Here, we aimed to analyze the effect of B1 antagonism on renal IRI and to study whether B1R knockout or antagonism could modulate the renal expression of pro and anti-inflammatory molecules. To this end, mice were subjected to 45 minutes ischemia and reperfused at 4, 24, 48 and 120 hours. Wild-type mice were treated intra-peritoneally with antagonists of either B1 (R-954, 200 mg/kg) or B2 receptor (HOE140, 200 mg/kg) 30 minutes prior to ischemia. Blood samples were collected to ascertain serum creatinine level, and kidneys were harvested for gene transcript analyses by real-time PCR. Herein, B1R antagonism ( R-954) was able to decrease serum creatinine levels, whereas B2R antagonism had no effect. the protection seen under B1R deletion or antagonism was associated with an increased expression of GATA-3, IL-4 and IL-10 and a decreased T-bet and IL-1b transcription. Moreover, treatment with R-954 resulted in lower MCP-1, and higher HO-1 expression. Our results demonstrated that bradykinin B1R antagonism is beneficial in renal IRI.
dc.languageeng
dc.publisherPublic Library Science
dc.relationPlos One
dc.rightsAcesso aberto
dc.titleBrabykinin B1 Receptor Antagonism Is Beneficial in Renal Ischemia-Reperfusion Injury
dc.typeArtigo


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