dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorArana-Chavez, V. E.
dc.creatorKatchburian, Eduardo [UNIFESP]
dc.date.accessioned2016-01-24T12:30:22Z
dc.date.accessioned2022-10-07T21:23:02Z
dc.date.available2016-01-24T12:30:22Z
dc.date.available2022-10-07T21:23:02Z
dc.date.created2016-01-24T12:30:22Z
dc.date.issued1997-07-01
dc.identifierAnatomical Record. New York: Wiley-liss, v. 248, n. 3, p. 332-338, 1997.
dc.identifier0003-276X
dc.identifierhttp://repositorio.unifesp.br/handle/11600/25745
dc.identifier10.1002/(SICI)1097-0185(199707)248:3<332
dc.identifierWOS:A1997XH46900005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4029037
dc.description.abstractBackground: Mature odontoblasts possess junctional structures constituted by adherens, gap, and tight junctions. Although adherens and gap junctions appear early between odontoblasts, there is no information on the appearance and development of tight junctions between odontoblasts. in this study, we have examined freeze-fracture replicas of early dentinogenesis to study the development of tight junctions between odontoblasts and to determine whether these junctions are of zonular or macular type,Methods: Upper first molar tooth germs of Wistar rats between 1 and 3 days old were fixed in buffered 4% glutaraldehyde/4% formaldehyde and subsequently cryoprotected with cacodylate-buffered glycerol, Freeze-fracture replicas were obtained in a Balzers 301 apparatus, and early stages of dentinogenesis were examined in a Jeol 100 CX II electron microscope,Results: in the stage of early dentine matrix prior to mineralization, odontoblasts exhibit only gap junctions, With the progression of development, the distal plasma membranes of odontoblasts show numerous short tight junctions formed by fused particles and grooves. in the stage of advanced mineralization, branched and continuous rows of fused particles or grooves constitute tight junctions of the focal or macular type,Conclusions: the present study shows that tight junctions of focal or macular type appear on distal plasma membrane of early odontoblasts during differentiation. Formation of tight junctions indicates the establishment of a distal membrane domain and maturation of odontoblasts. These events occur as mantle dentine formation ceases and circumpulpar dentine formation begins. (C) 1997 Wiley-Liss, Inc.
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationAnatomical Record
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.rightsAcesso aberto
dc.subjecttight junctions
dc.subjectodontoblasts
dc.subjectdentinogenesis
dc.subjectmineralization
dc.subjectodontoblast plasma membrane
dc.subjectdentine
dc.subjectfreeze fracture
dc.titleDevelopment of tight junctions between odontoblasts in early dentinogenesis as revealed by freeze-fracture
dc.typeArtigo


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