Artigo
Lepstospira interrogans shotgun phage display identified LigB as a heparin-binding protein
Fecha
2012-11-02Registro en:
Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 427, n. 4, p. 774-779, 2012.
0006-291X
WOS000311134700016.pdf
10.1016/j.bbrc.2012.09.137
WOS:000311134700016
Autor
Ching Ching, Ana Tung
Favaro, Regiane Degan
Lima, Swiany Silveira
Muniz Chaves, Agtha de Alencar
Lima, Marcelo Andrade de [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Estima Abreu, Patricia A.
Ho, Paulo Lee
Institución
Resumen
LigB is an adhesin from pathogenic Leptospira that is able to bind to extracellular matrix and is considered a virulence factor. A shotgun phage display genomic library was constructed and used for panning against Heparan Sulfate Proteoglycan (HSPG). A phage clone encoding part of LigB protein was selected in panning experiments and showed specific binding to heparin. To validate the selected clone, fragments of LigB were produced as recombinant proteins and showed affinity to heparin and to mammalian cells. Heparin was also able to reduce the binding of rLB-Ct to mammalian cells. Our data suggests that the glycosaminoglycan moiety of the HSPG is responsible for its binding and could mediate the attachment of the recombinant protein rLB-Ct. Thus, heparin may act as a receptor for Leptospira to colonize and to invade the host tissue. (C) 2012 Elsevier Inc. All rights reserved.